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Nucleic Acid MCQs | Nucleic Acid Multiple Choice Questions and Answers

(1) Identify the purine base of nucleic acids in the following
[A] Cytosine
[B] Thymine
[C] Uracil
[D] Adenine
Answer: Adenine
(2) Which of the following statements is true?
[A] Sugar component of a nucleotide is ribose
[B] Sugar component of a nucleotide is deoxyribose
[C] The bases in nucleotides are attached to a pentose sugar moiety by a glycosidic linkage
[D] The sugar molecule of the nucleotide is in L-configuration
Answer: The bases in nucleotides are attached to a pentose sugar moiety by a glycosidic linkage

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(3) What is the composition of nucleoside?
[A] a sugar + a phosphate
[B] a base + a sugar
[C] a base + a phosphate
[D] a base + a sugar + phosphate
Answer: a base + a sugar
(4) The sugar molecule in a nucleotide is
[A] Pentose
[B] Hexose
[C] Tetrose
[D] Triose
Answer: Pentose
(5) Arrangement of nucleotides in DNA can be seen by
[A] Ultracentrifuge
[B] X-Ray crystallography
[C] Light microscope
[D] Electron microscope
Answer: X-Ray crystallography
(6) One of the following nucleic acids has a left handed helix
[A] M-RNA
[B] T-RNA
[C] A-DNA
[D] Z-DNA
Answer: Z-DNA
(7) Which of the following is true about Z-DNA helix?
[A] It has alternating GC sequences
[B] It is a permanent conformation of DNA
[C] It tends to be found at the 3’ end of the genes
[D] It has fewer base pairs per turn than B-DNA
Answer: It has alternating GC sequences
(8) Which of the following statements is true?
[A] The template strand matches the sequence of the RNA transcript
[B] The two strands of DNA run parallel to each other
[C] G-C bonds are much more resistant to denaturation than A-T rich regions
[D] The common form of DNA is left handed
Answer: G-C bonds are much more resistant to denaturation than A-T rich regions
(9) Which structural feature is shared by both uracil and thymine?
[A] Both contain two keto groups
[B] Both contain one methyl group
[C] Both contain a five-membered ring
[D] Both contain three nitrogen atoms
Answer: Both contain two keto groups
(10) Histones
[A] are negatively-charged globular proteins
[B] contain both α-helix and β-pleated sheet
[C] have molecular weights in excess of 100,000
[D] contain high amounts of basic amino acids
Answer: contain high amounts of basic amino acids
(11) Which is a property of both viral genetic material and bacterial genetic material?
[A] Both can be double-stranded, circular DNA
[B] Both can be single-stranded, linear RNA
[C] Both can be compacted into inverted repeats
[D] Both can be compacted into plasmids
Answer: Both can be double-stranded, circular DNA
(12) Which are characteristics of bacterial genetic material?
[A] It is double-stranded and supercoiled
[B] It is single-stranded and relaxed
[C] It is circular and compacted into palindromes
[D] It is linear and attached to a protein scaffold
Answer: It is double-stranded and supercoiled
(13) Assume that DNA molecules are studied in a variety of organisms and found to have the following properties. Which property would be consistent with the hypothesis that genetic material is composed of DNA?
[A] DNA in all organisms is composed of the same nucleotides
[B] DNA in an organism remains constant as the organism ages
[C] DNA from two different organisms has the same base composition
[D] DNA is different in two different cells of the same organism
Answer: DNA in an organism remains constant as the organism ages
(14) A new virus, virus X, is isolated and studied. Which molecule is most likely to be the genetic material of virus X?
[A] a linear DNA molecule containing 10,000 base-pairs
[B] a linear RNA molecule containing plasmids
[C] a circular DNA molecule containing nucleosomes
[D] a circular RNA molecule with a molecular weight of 10 billion
Answer: a linear DNA molecule containing 10,000 base-pairs
(15) Which describes the structural properties of a eukaryotic chromosome?
[A] It contains a double-helix composed of nucleosomes each with four million base-pairs
[B] It is one double-stranded linear DNA molecule bound to proteins for compaction
[C] It is arranged into a 30 nm fiber which is attached to the cell membrane
[D] It contains linker regions which are arranged into loops and coiled to form a helix
Answer: It is one double-stranded linear DNA molecule bound to proteins for compaction
(16) If the following experimental results were obtained, which would be evidence against DNA being the genetic material?
[A] The base composition of DNA is the same in two different cells of the same organism
[B] The viral components labeled with 32P remain outside the cell during a successful infection
[C] The transforming factor isolated from a virulent bacterium is sensitive to deoxyribonucleases
[D] The base composition of DNA in an organism remains constant as the organism ages
Answer: The viral components labeled with 32P remain outside the cell during a successful infection
(17) Which is a characteristic of eukaryotic genetic material?
[A] Eukaryotic genetic material consists of supercoiled circular DNA molecules complexed with proteins into chromosomes
[B] Eukaryotic genetic material consists of relaxed linear DNA molecules complexed with RNA into a 30 nm fiber
[C] Eukaryotic genetic material is compacted by wrapping the double-helix around histone proteins to form nucleosomes
[D] Eukaryotic genetic material is compacted by folding linker regions around non-histone proteins to form a scaffold
Answer: Eukaryotic genetic material is compacted by wrapping the double-helix around histone proteins to form nucleosomes
(18) Which property is shared by the five histone proteins?
[A] They are acidic proteins with relatively large molecular weights
[B] They are multimeric proteins with high levels of Arg and Lys
[C] They are fibrous proteins with high amounts of α-helix
[D] They are globular proteins with a positive charge
Answer: They are globular proteins with a positive charge
(19) Which characteristic will be shared when comparing the single 5’-(GA)20-3’ strand to another single-stranded DNA molecule with the sequence 5’-(AT)20-3’?
[A] Both contain a palindromic sequence
[B] Both can form the same secondary structures
[C] Both could hybridize to the same RNA molecule
[D] Both will have the same overall charge
Answer: Both will have the same overall charge
(20) Which of the following double-stranded DNA molecules would denature at about the same temperature as the double-stranded molecule containing a 5’-(GA)20-3’ strand?
[A] a molecule which contains a (GC)20 strand
[B] a molecule which contains a (TA)20 strand
[C] a molecule which contains a (GACT)10 strand
[D] a molecule which contains a (GGGA)10 strand
Answer: a molecule which contains a (GACT)10 strand

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