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Nucleic Acid Quiz | Nucleic Acid Objective Type Questions and Answers

(21) Which component is found in both guanosine and uridine?
[A] Both contain an aldohexose
[B] Both contain three hydroxyl groups
[C] Both contain a 1’,9 - bond
[D] Both contain a pyranose
Answer: Both contain three hydroxyl groups
(22) Which property is shared by both CTP and dTDP?
[A] Both contain the same sugar
[B] Both contain the same charge at cellular pH
[C] Both contain a planar six-membered ring
[D] Both contain phosphodiester bonds
Answer: Both contain a planar six-membered ring

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(23) Which is a general property of both nucleosides and nucleotides?
[A] Both contain a pentose in the form of a furanose
[B] Both contain at least one 5’-phosphate group
[C] Both contain a nitrogenous base that forms covalent H-bonds
[D] Both contain a hemiacetal or hemiketal bond
Answer: Both contain a pentose in the form of a furanose
(24) Which characteristic does this double-stranded molecule have when it forms a B- DNA structure?
[A] The two strands will have parallel orientation and identical sequences
[B] The helix will be right-handed with 12 base-pairs per turn
[C] Every base-pair will contain one purine and one pyrimidine
[D] There are both covalent and non-covalent bonds between the two chains
Answer: Every base-pair will contain one purine and one pyrimidine
(25) Which of the following double-stranded DNA molecules would denature at a lower temperature than the 40 base-pair double-stranded molecule described above?
[A] a 40 base-pair molecule in which 25% of the bases are adenines
[B] a 30 base-pair molecule in which 40% of the bases are guanines
[C] a 20 base-pair molecule in which 10% of the bases are thymines
[D] a 10 base-pair molecule in which 20 % of the bases are cytosines
Answer: a 10 base-pair molecule in which 20 % of the bases are cytosines
(26) Which characteristic will this double-stranded DNA molecule share with a double-stranded RNA molecule of the same size?
[A] Both will have secondary structure
[B] Both will contain inverted repeats
[C] Both will be degraded by base
[D] Both will contain four types of base-pairs
Answer: Both will have secondary structure
(27) Which will be a characteristic of this one single strand?
[A] The single-stranded chain will contain both ribose and deoxyribose
[B] The single-stranded chain will contain both purines and pyrimidines
[C] The single-stranded chain will contain one 5’-end and one 3’-end
[D] The single-stranded chain will contain multiple phosphodiester bonds each linking a 2’-carbon and a 5’-carbon
Answer: The single-stranded chain will contain one 5’-end and one 3’-end
(28) Which of the following are not the components of RNA?
[A] Thymine
[B] Adenine
[C] Guanine
[D] Cytosine
Answer: Thymine
(29) What is the composition of nucleotide?
[A] a sugar + a phosphate
[B] a base + a sugar
[C] a base + a phosphate
[D] a base + a sugar + phosphate
Answer: a base + a sugar + phosphate
(30) Which of the following is true about phosphodiester linkage?
[A] 5’-phosphate group of one nucleotide unit is joined to the 3’-hydroxyl group of the next nucleotide
[B] 3’-phosphate group of one nucleotide unit is joined to the 5’-hydroxyl group of the next nucleotide
[C] 5’-phosphate group of one nucleotide unit is joined to the 5’-hydroxyl group of the next nucleotide
[D] 3’-phosphate group of one nucleotide unit is joined to the 3’-hydroxyl group of the next nucleotide
Answer: 5’-phosphate group of one nucleotide unit is joined to the 3’-hydroxyl group of the next nucleotide
31 Which of the following is false about purine and pyrimidine bases?
[A] They are hydrophobic and relatively insoluble in water at the near-neutral pH of the cell
[B] At acidic or alkaline pH the bases become charged and their solubility in water increases
[C] Purines have two rings in their structure, but pyrimidine bases have only one ring
[D] At acidic or alkaline pH the bases become charged and their solubility in water decreases
Answer: At acidic or alkaline pH the bases become charged and their solubility in water decreases
32 Building blocks of nucleic acids are
[A] Nucleotides
[B] Nucleosides
[C] Amino acids
[D] Histones
Answer: Nucleotides
33 Which of the following leads to disruption of nucleosomal structure?
[A] Acetylation
[B] Carboxylation
[C] Phosphorylation
[D] Methylation
Answer: Methylation
34 Which of the following statements is not true about RNA?
[A] Does not have a double stranded structure
[B] Thymine is present
[C] Does not obey Chargaff’s rule
[D] The sugar contained in RNA is a ribose
Answer: Thymine is present
35 Which component is found in both adenosine and deoxycytidine?
[A] Both contain a pyranose
[B] Both contain a 1,1’-N-glycosidic bond
[C] Both contain a pyrimidine
[D] Both contain a 3’-OH group
Answer: Both contain a 3’-OH group
36 Which property is shared by both GDP and AMP?
[A] Both contain the same charge at neutral pH
[B] Both contain the same number of phosphate groups
[C] Both contain the same purine
[D] Both contain the same furanose
Answer: Both contain the same furanose
37 Which characteristic is shared by purines and pyrimidines?
[A] Both contain two heterocyclic rings with aromatic character
[B] Both can form multiple non-covalent hydrogen bonds
[C] Both exist in planar configurations with a hemiacetal linkage
[D] Both exist as neutral zwitterions under cellular conditions
Answer: Both can form multiple non-covalent hydrogen bonds
38 Which characteristic is shared by both adenine and cytosine?
[A] Both contain one methyl group
[B] Both are anomeric
[C] Both contain one keto group
[D] Both are heterocyclic
Answer: Both are heterocyclic
39 Which characteristic is found in both purines and pyrimidines?
[A] They both have aromatic rings that undergo substantial tautomerization at neutral pH
[B] They both are weak bases that can be positively charged at neutral pH
[C] They both have multiple pKa values that result in zwitterion forms
[D] They both can form stable N-glycosidic bonds with β D-ribofuranose
Answer: They both can form stable N-glycosidic bonds with β D-ribofuranose
40 Which force can stabilize a DNA double-helix?
[A] Hydrophobic bases are found in the interior of the helix where each base- pair is stabilized by the same number of hydrogen bonds
[B] Hydrophilic sugar-phosphate groups are found on the exterior of the helix where they can interact with water
[C] Non-covalent N-glycosidic bonds can form between nitrogenous bases in opposite strands in the helix
[D] Covalent base-stacking interactions can occur between adjacent bases within the same strand in the helix
Answer: Hydrophilic sugar-phosphate groups are found on the exterior of the helix where they can interact with water

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