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Protein Folding Quiz | Protein Folding Multiple Choice Questions(MCQs) with Answers

(1) In a living organism, strong interactions between molecules are formed by a certain type of bond. Name that?
[A] Hydrogen bonding
[B] Covalent bond
[C] Electrostatic interactions
[D] Van der Waals forces
Answer: Covalent bond
(2) Mark the correct statement which depicts the correct statement of protein assay?
[A] To determine the amount of protein
[B] To characterize the function of the protein
[C] To determine the structure of the protein
[D] To determine the sequence of the protein
Answer: To determine the amount of protein

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(3) Proteolytic enzyme trypsin cleaves protein at which of these specific sites?
[A] Carboxyl side of Alanine, Glycine
[B] Carboxyl side of Lysin or Arginine
[C] Carboxyl side of aromatic amino acid
[D] The amino side of aromatic amino acid
Answer: Carboxyl side of Lysin or Arginine
(4) Which one of the following reagent is also known a Sanger’s reagent?
[A] 1-fluoro-2,4-dinitrobenzene (FDNB)
[B] Phenylisothiocyanate
[C] Cyanogen bromide
[D] β-mercaptoethanol
Answer: 1-fluoro-2,4-dinitrobenzene (FDNB)
(5) Among all the heat shock proteins which one is known as chaperonins?
[A] Hsp70
[B] Hsp32
[C] Hsp60
[D] Hsp30
Answer: Hsp60
(6) Which of these heat shock proteins prevent protein misfolding and maintain polypeptide chain in the unfolded state.
[A] Hsp60
[B] Hsp28
[C] Hsp70
[D] Hsp32
Answer: Hsp70
(7) Which of the following name is given to molecular chaperones?
[A] Allosteric protein
[B] Heat shock protein
[C] Denaturation protein
[D] Ribonuclease
Answer: Heat shock protein
(8) Which sentence is INCORRECT regarding molten globule state of protein?
[A] Hydrophobic interaction among non-polar residues
[B] The collapsed state
[C] It is less compact
[D] The denatured state
Answer: The denatured state
(9) Which of them contains all the information required to fold the polypeptide chain in its 3-D structure?
[A] DNA sequences
[B] RNA
[C] Histone protein
[D] Amino acid sequences
Answer: Amino acid sequences
(10) Protein folding is a process in which a polypeptide folds in to ___________
[A] 2-D structure
[B] Globular form
[C] 3-D structure
[D] Linear form
Answer: 3-D structure
(11) The characteristic strong absorbance of 280nm of proteins is due to
[A] Tryptophan, tyrosine and phenylalanine can absorb at 280 nm
[B] Tryptophan is responsible for the absorbance
[C] Only Tryptophan and tyrosine can absorb at 280 nm
[D] All amino acids can absorb at 280nm
Answer: Tryptophan, tyrosine and phenylalanine can absorb at 280 nm
(12) All the following statements are true except
[A] Ornithine and citrulline are uncommon aminoacids
[B] Ornithine is an intermediates in Urea cycle
[C] Ormithine and citrulline are uncommon amino acids present in many proteins
[D] Both ornithine and citrulline are intermediates in urea cycle
Answer: Both ornithine and citrulline are intermediates in urea cycle
(13) Tri-peptide consists
[A] 3 amino acids and 3 peptide bonds
[B] 2 amino acids and 3 peptide bonds
[C] 3 amino acids and 2 peptide bonds
[D] 3 amino acids and 4 peptide bonds
Answer: 3 amino acids and 2 peptide bonds
(14) Peptide bonds between amino acids are highly stable and have a half-life of seven years in intracellular condition. This is due to
[A] High activation energy required for hydrolysis
[B] Low activation energy required for hydrolysis
[C] Peptide bond is a covalent bond
[D] Peptide bond is rigid and planar
Answer: High activation energy required for hydrolysis
(15) 4-hydroxy proline is present in
[A] Collagen
[B] Plant cell wall
[C] Keratin
[D] Both plant cell wall and collagen
Answer: Both plant cell wall and collagen
(16) The formation of cystine occurs at
[A] ER
[B] Golgi
[C] Cytosol
[D] Mitochondria
Answer: ER
(17) Which of the following interaction contributes most in protein folding
[A] Covalent bond
[B] Ionic bond
[C] Hydrophobic interaction
[D] Vander walls interaction
Answer: Hydrophobic interaction
(18) Which of the following aminoacid has pKa near neutrality
[A] Tryptophan
[B] Arginine
[C] Histidine
[D] Asparagine
Answer: Histidine
(19) Desmosine is an unusual amino acid found in
[A] Myosin
[B] Elastin
[C] Troponin
[D] Actin
Answer: Elastin
(20) How many small peptides are formed upon cleavage by trypsin if a protein has 5 lysine residues
[A] 4
[B] 5
[C] 6
[D] 7
Answer: 6

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