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Sterilization MCQs | Sterilization Multiple Choice Questions and Answers

(1) The process in which all living cells, spores and viruses are completely destroyed from an object is called _____.
[A] Disinfection
[B] Pasteurization
[C] Sterilization
[D] Antisepsis
Answer: Sterilization
(2) _______ is an antiseptic.
[A] Chlorine
[B] Copper Sulphate
[C] Ozone
[D] 70% ethanol
Answer: 70% ethanol

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(3) Same DNA sequence may code for more than one protein by ______
[A] Gene splitting
[B] RNA splitting
[C] Alternative splicing of RNA
[D] None
Answer: Alternative splicing of RNA
(4) Glucose that is taken in by a bacterial cell is made to stay within the cell by _______.
[A] Attaching to membranes
[B] Immediate utilization
[C] Phosphorylation
[D] None of the above
Answer: Phosphorylation
(5) _____ is a predator bacterium that infects other gram negative bacteria.
[A] Haemophilus influenza
[B] Pseudomonas putida
[C] Proteus mirabilis
[D] Bdellovibrio
Answer: Bdellovibrio
(6) Which of the following is not a type of sterilization?
[A] Batch
[B] Continuous
[C] Filter
[D] Submerged
Answer: Submerged
(7) What do you mean by sterilization?
[A] Purification of products
[B] Recovery of products
[C] Elimination of contamination
[D] None Of The Above
Answer: Elimination of contamination
(8) The highest feasible temperature for batch sterilization is ________
[A] 124°C
[B] 120°C
[C] 121°C
[D] None of these
Answer: 121°C
(9) The destruction of microorganisms by moist heat is described by ______________
[A] Zero-order reaction
[B] First-order reaction
[C] Third-order reaction
[D] Second-order reaction
Answer: First-order reaction
(10) The long exposure of batch sterilization may lead to ____________
[A] Purification of media
[B] Recovery of media
[C] Degradation
[D] Good quality of product
Answer: Degradation
(11) What is the advantage of batch sterilization over continuous sterilization?
[A] Superior maintenance of medium quality
[B] Ease of scale-up
[C] Automatic control
[D] Lower equipment costs
Answer: Lower equipment costs
(12) The equation for Del factor is _____________
[A] ∇ = ln (N0/Nt)
[B] ∇ = ln (Nt/N0)
[C] ∇ = ln (N0)
[D] None of the above
Answer: ∇ = ln (N0/Nt)
(13) The Del Factor decreases as the initial number of organisms _____________
[A] Decreases
[B] Increases
[C] Remains constant
[D] Becomes zero
Answer: Decreases
(14) How long does it take for the autoclave to complete its cycle?
[A] 30-35 minutes
[B] 50 min to 1 hr
[C] 15-20 minutes
[D] 10-15 minutes
Answer: 15-20 minutes
(15) Which scientists introduced the term ‘Del factor’?
[A] Deindoerfer and Humphrey
[B] Jacob and Monod
[C] Banks and Corbett
[D] None of these
Answer: Deindoerfer and Humphrey
(16) Which of the following reaction occurs during the sterilization which results in browning of media?
[A] Sandmeyer reaction
[B] Maillard reaction
[C] Cannizzaro reaction
[D] Gattermann reaction
Answer: Maillard reaction
(17) If the foreign microorganism invades the fermentation, which of the following is not likely to occur?
[A] The medium will not allow the growth of contaminant
[B] The contaminant may outgrow the production organism
[C] The contaminant may contaminate the final product
[D] The contaminant may degrade the final product
Answer: The medium will not allow the growth of contaminant
(18) UHT sterilization involves high temperature exposure of objects for _____.
[A] 1 – 3 seconds
[B] 1 – 3 minutes
[C] 1 – 3 hours
[D] 10 – 30 hours
Answer: 1 – 3 seconds
(19) _____ RNA polymerase has a single subunit.
[A] E. coli
[B] Bacillus subtilis
[C] T7
[D] λ phage
Answer: T7
(20) The best form of DNA to transform E. coli is ______.
[A] Linear
[B] Covalently closed
[C] Nicked circles
[D] None Of The Above
Answer: Covalently closed

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