As the two strands of the double helix are separated, the positive supercoiling interferes with the further unwinding of DNA

Q. As the two strands of the double helix are separated, the positive supercoiling interferes with the further unwinding of DNA. Which of the following enzyme makes a break in a strand of DNA to release the supercoiling and facilitate the replication to occur?

A.            DnaA protein

B.            Single-strand binding protein

C.            DNA polymerase

D.            Topoisomerase

Answer: Topoisomerase

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