![As the two strands of the double helix are separated, the positive supercoiling interferes](https://www.gkseries.com/blog/wp-content/uploads/2022/02/As-the-two-strands-of-the-double-helix-are-separated-the-positive-supercoiling-interferes.png)
Q. As the two strands of the double helix are separated, the positive supercoiling interferes with the further unwinding of DNA. Which of the following enzyme makes a break in a strand of DNA to release the supercoiling and facilitate the replication to occur?
A. DnaA protein
B. Single-strand binding protein
C. DNA polymerase
D. Topoisomerase
Answer: Topoisomerase