Q. Proteins are separated in an SDS-PAGE experiment on the basis of their positively charged side chainsmolecular weightnegatively charged side chainsdifferent isoelectric points Answer: molecular weight

Q. Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in both proteins migrate to the anodehistones migrate to the anode and myoglobin migrates to the cathodehistones migrate to the cathode and myoglobin migrates to the anodeboth proteins migrate to the ...

Q. Proteins can be visualized directly in gels by staining them with the dyeusing electron microscope onlymeasuring their molecular weightnone of these Answer: staining them with the dye

Q. In SDS-PAGE, separation is based on molecular weightshapechargeall of the above Answer: molecular weight

Q. The electrophoresis technique that used isoelectric focusing is AGEPFGE2D-PAGESDS-PAGE Answer: 2D-PAGE

Q. What is the role of SDS in SDS-PAGE? protein denaturing and imparting net negative chargeimparting overall negative charge to the proteinimparting equal mass to all proteinsprotein unfolding and imparting net positive charge Answer: protein denaturing and imparting net negative charge

Q. Which is the technique suited for the separation of large DNA fragments? AGEPAGEPFGESDS-PAGE Answer: PFGE

Q. Electrophoresis cell or electrophoresis apparatus consists of power pack and electrophoresis unitElctrophoresis unit and DNA separatorbuffer chamber and Elctrophoresis unitGel, buffer chamber and power pack Answer: power pack and electrophoresis unit

Q. The most common type of gel used for DNA separation is AgarPolyacrylamideAgaroseAll of the above Answer: Agarose

Q. Which of the following statement is true regarding migration of bio molecules? the rate of migration is directly proportional to the currentthe rate of migration is inversely proportional to currentthe rate of migration is directly proportional to the resistance of the mediumLow voltage is ...

Q. The technique electrophoresis, for the separation of charged molecules was developed by TswettSvedbergTiseliusSanger Answer: Tiselius

Q. In electrophoresis, DNA will migrate towards cathode or positive electrodeanode or negative electrodecathode or negative electrodeanode or positive electrode Answer: anode or positive electrode