Q. The speed of migration of ions in an electric field depends on magnitude of charge and mass of moleculesmagnitude of charge and shape of moleculesshape and size of the moleculemagnitude of charge, shape and mass of molecules Answer: magnitude of charge and shape of molecules

Q. Which of the following will migrate faster? The condition is the molecular weight of the following is equal. Nicked circular DNADouble stranded DNASingle stranded DNASupercoiled circular DNA Answer: Supercoiled circular DNA

Q. If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length how many bands would be seen after electrophoresis? 345Any of the above Answer: 4

Q. What cannot be a reason for using electrophoresis? Comparing two sets of DNAOrganizing DNA by shape of backboneOrganizing DNA fragments from largest to smallestOrganizing DNA in order we can see Answer: Organizing DNA by shape of backbone

Q. What does the electrophoresis apparatus consist of? Gel, buffer chamber and fire packBuffer chamber and electrophoresis unitPower pack and electrophoresis unitElectrophoresis unit and gel separator Answer: Power pack and electrophoresis unit

Q. Glycerol is added to protein samples before they are loaded to the ‘wells’ of PAGE. The function of glycerol is to _______. Stabilize protein structureProvide density to the sampleHelps to bind SDS to the proteinHelps to reduce disulfide bonds by β-mercaptoethanol Answer: Provide density ...

Q. In SDS-PAGE of protein separation, one SDS molecule will binds to __________. Every amino acidEvery two amino acidsEvery three amino acidsEvery Four amino acids Answer: Every two amino acids

Q. Electrophoresis is not used for the separation of ________. Nucleic acidsProteinsAmino acidsLipids Answer: Lipids

Q. The ratio of velocity (‘v’) of biomolecule in a medium under constant electric field (‘E’) is called ‘Electrophoretic mobility’ denoted as ‘µ’. ‘µ’ is mathematically expressed as: µ = E/vµ = 1/(Ev)µ = VEµ = v/E Answer: µ = v/E

Q. Function of β-mercaptoethanol in SDS-PAGE is__________. To give negative charges to amino acids in the proteinsFor the oxidation of disulfide bonds in the proteinsFor breaking hydrogen bonds in the proteinsFor the reduction of disulfide bonds in the proteins Answer: For the reduction of ...

Q. In electrophoresis, the electrophoretic mobility (‘µ’) is determines the characteristics of migration of different biomolecules. Which of the following is not having any influence in ‘µ’? Size of moleculeShape of moleculeMolecular weightStereochemistry of molecule Answer: Stereochemistry ...

Q. In native-PAGE the separation of protein is influenced by ___________. Charge of proteinSize of proteinpI of proteinBoth (a) and (b) Answer: Both (a) and (b)