Q. For the separation of DNA by electrophoresis, which of the following method is commonly used? Agarose – verticalAgarose – horizontalPAGE – verticalPAGE – horizontal Answer: Agarose – horizontal

Q. When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as: E = V/dE = (1/V) x qE = (Vd)/qNone of these Answer: E = V/d

Q. Pulse field gel electrophoresis was developed by ____________ Collins and JohnKary MullisPatrick O’ FarrellSchwartz and Cantor Answer: Schwartz and Cantor

Q. Pulse field gel electrophoresis separates DNA molecules of size ___________ 10 – 20 bp20 – 30 Kb30 – 50 Kb40 – 50 bp Answer: 30 – 50 Kb

Q. The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA? Stacked between histone moleculesBinds to the nucleotide baseIntercalated between the stacked basesBinds to the phosphodiester backbone Answer: Intercalated between the stacked bases

Q. The polymerization of the gel used in PAGE occurs between polyacrylamide and____________ N, N – acrylamideBisacrylamideN – methyleneacrylamideN, N – methylene bisacrylamide Answer: N, N – methylene bisacrylamide

Q. Which of the following cannot be used for the separation of nucleic acids? SDS – PAGEPAGENorthern blottingPAGE Answer: SDS – PAGE

Q. When is electrophoresis not used? Separation of proteinsSeparation of amino acidsSeparation of LipidsSeparation of nucleic acids Answer: Separation of Lipids

Q. Which of the following factors does not influence electrophoretic mobility? Molecular weightShape of moleculeSize of moleculeStereochemistry of molecule Answer: Stereochemistry of molecule

Q. The electrophoretic mobility denoted as µ is mathematically expressed as: VEV/EE/V1/EV Answer: V/E

Q. Agarose can be extracted from which of the following? Lycazusican esculentumFicum benghalensisGracilaria esculentusAgrostis stolonifera Answer: Gracilaria esculentus

Q. Which of the following statements is true about migration of biomolecules? The rate of migration is directly proportional to the resistance of mediumRate of migration is directly proportional to currentLow voltage is used for separation of high mass moleculesRate of migration is inversely ...