In SDS-PAGE, the protein sample is first

Q. In SDS-PAGE, the protein sample is first

1. treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
2. fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent
3. treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
4. none of the above

Answer: treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis