Q. Electrophoresis is not used for the separation of ________. Nucleic acidsProteinsAmino acidsLipids Answer: Lipids

Q. The ratio of velocity (‘v’) of biomolecule in a medium under constant electric field (‘E’) is called ‘Electrophoretic mobility’ denoted as ‘µ’. ‘µ’ is mathematically expressed as: µ = E/vµ = 1/(Ev)µ = VEµ = v/E Answer: µ = v/E

Q. Function of β-mercaptoethanol in SDS-PAGE is__________. To give negative charges to amino acids in the proteinsFor the oxidation of disulfide bonds in the proteinsFor breaking hydrogen bonds in the proteinsFor the reduction of disulfide bonds in the proteins Answer: For the reduction of ...

Q. In electrophoresis, the electrophoretic mobility (‘µ’) is determines the characteristics of migration of different biomolecules. Which of the following is not having any influence in ‘µ’? Size of moleculeShape of moleculeMolecular weightStereochemistry of molecule Answer: Stereochemistry ...

Q. In native-PAGE the separation of protein is influenced by ___________. Charge of proteinSize of proteinpI of proteinBoth (a) and (b) Answer: Both (a) and (b)

Q. The role of urea in PAGE separation of DNA is to _______. Act as anionAct as cationHelps to denature the DNAProvide buffer stability of the gel Answer: Helps to denature the DNA

Q. In a native PAGE, proteins are separated on the basis of net negative chargenet charge and sizenet positive charges sizenet positive charge Answer: net charge and size

Q. In an SDS-PAGE proteins are denatured by the SDSproteins have the same charge-to-mass ratiosmaller proteins migrate more rapidly through the gelall of the above Answer: all of the above

Q. In SDS-PAGE, the protein sample is first treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresisfractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergenttreated with a oxidizing agent and then with ...

Q. In isoelectric focusing, proteins are separated on the basis of their relative content of positively charged residue onlyrelative content of negatively charged residue onlysizerelative content of positively and negatively charged residue Answer: relative content of positively and ...