Q. For the better resolution of minute protein bands in SDS-PAGE, which of the following staining method is advised? CBB StainingSilver stainingAvidin stainingAll of these Answer: Silver staining

Q. Which of the following is used as a ‘tracking dye’ in SDS-PAGE of protein? Bromophenol blueXylene cyanolOrange GAll of these Answer: All of these

Q. The role of APS in SDS PAGE is to________. Act as a catalyst in the polymerization of acrylamideAct as a source of free radicalsAct as a bridge between acrylamide and bis-acrylamideAct as a pore builder in the polymerized gel Answer: Act as a source of free radicals

Q. The pH of (i) stacking, (ii) resolving gel and (iii) tank buffer in SDS PAGE is_________ respectively. (i). 8.30 (ii) 8.80 (iii) 6.80(i). 6.80 (ii) 8.80 (iii) 8.30(i). 8.30 (ii) 6.80 (iii) 8.80Any of the above Answer: (i). 6.80 (ii) 8.80 (iii) 8.30

Q. The main advantage of discontinuous buffer system in SDS and Native PAGE is________. Conformation of protein is conservedConstantly maintain the charge of proteinsAssist in migration of proteinEnhance resolution of separation Answer: Enhance resolution of separation

Q. In SDS-PAGE, migration of protein is effected by ____________. Charge of proteinSize of proteinNet charge of proteinAll of these Answer: Size of protein

Q. Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________. An anionic detergentA cationic detergentA non-ionic detergentAn anion exchanger Answer: An anionic detergent

Q. For the separation of DNA by electrophoresis, which of the following method is commonly used? Agarose – verticalAgarose – horizontalPAGE – verticalPAGE – horizontal Answer: Agarose – horizontal

Q. When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as: E = V/dE = (1/V) x qE = (Vd)/qNone of these Answer: E = V/d

Q. Pulse field gel electrophoresis was developed by ____________ Collins and JohnKary MullisPatrick O’ FarrellSchwartz and Cantor Answer: Schwartz and Cantor