The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified

Q. The product of complete digestion of the plasmid shown below with EcoRI and HaeIII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and [a-32P]-dATP in a suitable reaction buffer. The product thus obtained was purified and subjected to gel electrophoresis followed by autoradiography.

The number of bands that will appear on the X-ray film is   

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