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Which of the following are not performed in lyophilization?

Which of the following are not performed in lyophilization?

Q. Which of the following are not performed in lyophilization?

  1. bacterial sample is dehydrated
  2. cell suspension is frozen at -60 degree to -78 degree C
  3. vials are connected to high-vacuum line
  4. agar slant is covered with mineral oil

Answer: agar slant is covered with mineral oil

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