Electrophoresis MCQs | Electrophoresis Multiple Choice Questions and Answers

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Questions
1 Which technique separates charged particles using electric field?
A Hydrolysis
B Electrophoresis
C Protein synthesis
D Protein denaturing

Answer: Electrophoresis
2 The speed of migration of ions in electric field depends upon:
A Shape and size of molecule
B Magnitude of charge and shape of molecule
C Magnitude of charge shape and mass of molecule
D Magnitude of charge and mass of molecule

Answer: Magnitude of charge and shape of molecule
3 Which of the following statements is true about migration of biomolecules?
A The rate of migration is directly proportional to the resistance of medium
B Rate of migration is directly proportional to current
C Low voltage is used for separation of high mass molecules
D Rate of migration is inversely proportional to current

Answer: Rate of migration is directly proportional to current
4 Agarose can be extracted from which of the following?
A Lycazusican esculentum
B Ficum benghalensis
C Gracilaria esculentus
D Agrostis stolonifera

Answer: Gracilaria esculentus
5 The electrophoretic mobility denoted as µ is mathematically expressed as:
A VE
B V/E
C E/V
D 1/EV

Answer: V/E
6 Which of the following factors does not influence electrophoretic mobility?
A Molecular weight
B Shape of molecule
C Size of molecule
D Stereochemistry of molecule

Answer: Stereochemistry of molecule
7 When is electrophoresis not used?
A Separation of proteins
B Separation of amino acids
C Separation of Lipids
D Separation of nucleic acids

Answer: Separation of Lipids
8 Which of the following cannot be used for the separation of nucleic acids?
A SDS – PAGE
B PAGE
C Northern blotting
D PAGE

Answer: SDS – PAGE
9 The polymerization of the gel used in PAGE occurs between polyacrylamide and ____________
A N, N – acrylamide
B Bisacrylamide
C N – methyleneacrylamide
D N, N – methylene bisacrylamide

Answer: N, N – methylene bisacrylamide
10 The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA?
A Stacked between histone molecules
B Binds to the nucleotide base
C Intercalated between the stacked bases
D Binds to the phosphodiester backbone

Answer: Intercalated between the stacked bases
11 Pulse field gel electrophoresis separates DNA molecules of size ___________
A 10 – 20 bp
B 20 – 30 Kb
C 30 – 50 Kb
D 40 – 50 bp

Answer: 30 – 50 Kb
12 Pulse field gel electrophoresis was developed by ____________
A Collins and John
B Kary Mullis
C Patrick O’ Farrell
D Schwartz and Cantor

Answer: Schwartz and Cantor
13 When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as:
A E = V/d
B E = (1/V) x q
C E = (Vd)/q
D None of these

Answer: E = V/d
14 For the separation of DNA by electrophoresis, which of the following method is commonly used?
A Agarose – vertical
B Agarose – horizontal
C PAGE – vertical
D PAGE – horizontal

Answer: Agarose – horizontal
15 Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________.
A An anionic detergent
B A cationic detergent
C A non-ionic detergent
D An anion exchanger

Answer: An anionic detergent
16 In SDS-PAGE, migration of protein is effected by ____________.
A Charge of protein
B Size of protein
C Net charge of protein
D All of these

Answer: Size of protein
17 The main advantage of discontinuous buffer system in SDS and Native PAGE is ________.
A Conformation of protein is conserved
B Constantly maintain the charge of proteins
C Assist in migration of protein
D Enhance resolution of separation

Answer: Enhance resolution of separation
18 The pH of (i) stacking, (ii) resolving gel and (iii) tank buffer in SDS PAGE is _________ respectively.
A (i). 8.30 (ii) 8.80 (iii) 6.80
B (i). 6.80 (ii) 8.80 (iii) 8.30
C (i). 8.30 (ii) 6.80 (iii) 8.80
D Any of the above

Answer: (i). 6.80 (ii) 8.80 (iii) 8.30
19 The role of APS in SDS PAGE is to________.
A Act as a catalyst in the polymerization of acrylamide
B Act as a source of free radicals
C Act as a bridge between acrylamide and bis-acrylamide
D Act as a pore builder in the polymerized gel

Answer: Act as a source of free radicals
20 The ‘tracking dye’ used in SDS PAGE will be ______.
A Anionic
B Cationic
C Non-ionic
D Amphipathic

Answer: Anionic

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