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Free download in PDF Genetic Engineering Multiple Choice Questions and Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams. These short solved questions or quizzes are provided by Gkseries.
(1)
The first crop plant genome sequenced
[A]
Maize
[B]
Wheat
[C]
Rice
[D]
Barley
(2)
Urea and Formamide can:
[A]
Increase Tm of DNA
[B]
Decrease the Tm of DNA
[C]
Helps faster re-annealing of DNA
[D]
Does not effects denaturation of DNA
Answer: Decrease the Tm of DNA
(3)
The first transgenic plant to be produced:
[A]
Rice
[B]
Maize
[C]
Cotton
[D]
Tobacco
(4)
Explants used for tissue culture can be taken from:
[A]
Bud
[B]
Root tip
[C]
Nodal stem segments
[D]
All of these
(5)
A low auxin:cytokinin ratio leads to:
[A]
Shoot formation
[B]
Root formation
[C]
Fruit formation
[D]
All of these
(6)
Klenow fragment is derived from:
[A]
DNA Pol-I
[B]
RNA Pol II
[C]
DNA Ligase
[D]
None Of The Above
(7)
The year of launching of Human Genome Project and completion of the rough draft of the sequence was in:
[A]
1992 and 2000
[B]
1990 and 2000
[C]
1989 and 2001
[D]
None Of The Above
(8)
The main difference between a plasmid and an episome is:
[A]
Plasmid is circular episome is linear
[B]
Episome is circular, plasmid is linear
[C]
Plasmid can be insert into host genome, episomes are not
[D]
Episomes can be inserted into the host genome
Answer: Episomes can be inserted into the host genome
(9)
What is the final product of the RNaseH method?
[A]
blunt ended dsDNA
[B]
staggered dsDNA at both ends
[C]
staggered dsDNA at 3’ end
[D]
staggered dsDNA at 5’ end
Answer: blunt ended dsDNA
(10)
The loop region is single stranded. It can be cleaved by using which enzyme?
[A]
Exonuclease
[B]
S1 nuclease
[C]
RNaseH
[D]
DNase
(11)
What is the second primer in the case of 5’ RACE?
[A]
Internal primer
[B]
Oligo-dA sequence
[C]
Adaptor-oligo-dT primer
[D]
Oligo-dT adaptor molecule
Answer: Adaptor-oligo-dT primer
(12)
DNA sequencing is done by
[A]
Maxam Gilbert method
[B]
Sanger dideoxy method
[C]
Both a and b
[D]
None of the above
(13)
DNA staining is done by
[A]
Cystal violet
[B]
Giemsa staining
[C]
Methylene blue
[D]
Feulgen staining
(14)
Use of alkaline phosphatase is to
[A]
remove terminal phosphates from 3’end
[B]
remove terminal phosphates from 5’end
[C]
remove terminal phosphate from both end
[D]
All of these
(15)
Taq polymerase is used in PCR because of its
[A]
low thermal stability
[B]
high fidelity
[C]
high speed
[D]
high thermal stability
Answer: high thermal stability
(16)
Nick translation is done by
[A]
DNA polymerase I
[B]
DNA polymerase II
[C]
DNA ligase
[D]
Kinase
(17)
Introduction of recombinant DNA into bacterial cell by using current is called
[A]
Transformation
[B]
Electroporation
[C]
Transformation
[D]
Tranduction
(18)
Telomeric sequences are found in
[A]
HAC
[B]
BAC
[C]
YAC
[D]
PAC
(19)
The uptake of plasmid DNA into bacterial cell is facilitated in the presence of
[A]
Calcium Chloride
[B]
Magnesium Chloride
[C]
Potassium Chloride
[D]
All Of These
(20)
Which of the following is not a restriction endonuclease?
[A]
Eco RI
[B]
DNA ligase
[C]
Hind III
[D]
None Of The Above
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