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Genetic Engineering MCQs | Genetic Engineering Multiple Choice Questions and Answers

(1) The first crop plant genome sequenced
[A] Maize
[B] Wheat
[C] Rice
[D] Barley
Answer: Rice
(2) Urea and Formamide can:
[A] Increase Tm of DNA
[B] Decrease the Tm of DNA
[C] Helps faster re-annealing of DNA
[D] Does not effects denaturation of DNA
Answer: Decrease the Tm of DNA

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(3) The first transgenic plant to be produced:
[A] Rice
[B] Maize
[C] Cotton
[D] Tobacco
Answer: Tobacco
(4) Explants used for tissue culture can be taken from:
[A] Bud
[B] Root tip
[C] Nodal stem segments
[D] All of these
Answer: All of these
(5) A low auxin:cytokinin ratio leads to:
[A] Shoot formation
[B] Root formation
[C] Fruit formation
[D] All of these
Answer: Shoot formation
(6) Klenow fragment is derived from:
[A] DNA Pol-I
[B] RNA Pol II
[C] DNA Ligase
[D] None Of The Above
Answer: DNA Pol-I
(7) The year of launching of Human Genome Project and completion of the rough draft of the sequence was in:
[A] 1992 and 2000
[B] 1990 and 2000
[C] 1989 and 2001
[D] None Of The Above
Answer: 1990 and 2000
(8) The main difference between a plasmid and an episome is:
[A] Plasmid is circular episome is linear
[B] Episome is circular, plasmid is linear
[C] Plasmid can be insert into host genome, episomes are not
[D] Episomes can be inserted into the host genome
Answer: Episomes can be inserted into the host genome
(9) What is the final product of the RNaseH method?
[A] blunt ended dsDNA
[B] staggered dsDNA at both ends
[C] staggered dsDNA at 3’ end
[D] staggered dsDNA at 5’ end
Answer: blunt ended dsDNA
(10) The loop region is single stranded. It can be cleaved by using which enzyme?
[A] Exonuclease
[B] S1 nuclease
[C] RNaseH
[D] DNase
Answer: S1 nuclease
(11) What is the second primer in the case of 5’ RACE?
[A] Internal primer
[B] Oligo-dA sequence
[C] Adaptor-oligo-dT primer
[D] Oligo-dT adaptor molecule
Answer: Adaptor-oligo-dT primer
(12) DNA sequencing is done by
[A] Maxam Gilbert method
[B] Sanger dideoxy method
[C] Both a and b
[D] None of the above
Answer: Both a and b
(13) DNA staining is done by
[A] Cystal violet
[B] Giemsa staining
[C] Methylene blue
[D] Feulgen staining
Answer: Feulgen staining
(14) Use of alkaline phosphatase is to
[A] remove terminal phosphates from 3’end
[B] remove terminal phosphates from 5’end
[C] remove terminal phosphate from both end
[D] All of these
Answer: All of these
(15) Taq polymerase is used in PCR because of its
[A] low thermal stability
[B] high fidelity
[C] high speed
[D] high thermal stability
Answer: high thermal stability
(16) Nick translation is done by
[A] DNA polymerase I
[B] DNA polymerase II
[C] DNA ligase
[D] Kinase
Answer: DNA polymerase I
(17) Introduction of recombinant DNA into bacterial cell by using current is called
[A] Transformation
[B] Electroporation
[C] Transformation
[D] Tranduction
Answer: Electroporation
(18) Telomeric sequences are found in
[A] HAC
[B] BAC
[C] YAC
[D] PAC
Answer: YAC
(19) The uptake of plasmid DNA into bacterial cell is facilitated in the presence of
[A] Calcium Chloride
[B] Magnesium Chloride
[C] Potassium Chloride
[D] All Of These
Answer: All Of These
(20) Which of the following is not a restriction endonuclease?
[A] Eco RI
[B] DNA ligase
[C] Hind III
[D] None Of The Above
Answer: DNA ligase

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