Genetic Engineering Quiz | Genetic Engineering Objective Type Questions and Answers

(21) A vector should have which of the following properties i) MCS ii) Small size iii) Multiple ori iv)low replication speed
[A] i, ii, iii
[B] ii, iii, iv
[C] i, ii, iv
[D] i, iii, iv
Answer: i, ii, iv
(22) The chromogenic substrate of X-gal is:
[A] 5-chloro-4-bromo-3indolyl-beta-D-galactoside
[B] 5-bromo-4-chloro-3indolyl-beta-D-galactoside
[C] 5-chloro-5-bromo-3indolyl-beta-D-galactoside
[D] 5-bromo-5-chloro-3indolyl-beta-D-galactoside
Answer: 5-bromo-4-chloro-3indolyl-beta-D-galactoside

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(23) Which of the following molecules is useful as a measure of relatedness beyond species level between organisms:
[A] mRNA
[B] tRNA
[C] rRNA
[D] miRNA
Answer: rRNA
(24) Indole -3-acetic acid is:
[A] A gibberellin
[B] A cytokinin
[C] An auxin
[D] All of these
Answer: An auxin
(25) Hairy root cultures are produced by using:
[A] Agrobacterium tumefaciens
[B] Agrobacterium rhizogenes
[C] Agrobacterium vitis
[D] All of these
Answer: Agrobacterium rhizogenes
(26) Polyhedrin protein is associated with one of the following viruses:
[A] HIV
[B] HSV
[C] NPV
[D] None Of The Above
Answer: NPV
(7) Which of the following is used as a fusogen:
[A] Polyethylene glycol
[B] CaMV
[C] Sendai Virus
[D] None Of The Above
Answer: Polyethylene glycol
(28) Restriction endonucleases are usually supplied in:
[A] 10% glycerol
[B] 30% glycerol
[C] 50% glycerol
[D] 80% glycerol
Answer: 50% glycerol
(29) Choose the incorrect statement for second DNA strand synthesis.
[A] The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol
[B] The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends
[C] These RNA fragments can be used as primers
[D] There is no portion of RNA left attached to the DNA strand
Answer: There is no portion of RNA left attached to the DNA strand
(30) What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?
[A] There are no chances of the synthesis of the second DNA strand
[B] Chance complementarity would take place
[C] Hairpin structure would be formed
[D] Hairpin structure is formed is not the final structure
Answer: There are no chances of the synthesis of the second DNA strand
31 Choose the correct statement with respect to the self priming method of cDNA synthesis.
[A] It is less preferred than RNaseH method
[B] A hairpin structure is formed with guarantee
[C] The sequence corresponding to the 5’ end is lost
[D] Reverse transcriptase is not used
Answer: The sequence corresponding to the 5’ end is lost
32 Choose the incorrect statement for the method homopolymer tailing.
[A] The first step is the RNA: DNA hybrid synthesis
[B] Terminal transferase is used for the addition of nucleotides on 3’ end
[C] Terminal transferase adds only at DNA strands
[D] None of the above
Answer: Terminal transferase adds only at DNA strands
33 Choose the correct statement for RACE.
[A] It stands for Random Amplification of cDNA ends
[B] It is for cloning particular cDNA ends
[C] It is only of one type, which is 5’ RACE
[D] Sequence data is not available in any case
Answer: It is only of one type, which is 5’ RACE