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Principles and Processes of Biotechnology MCQs | Principles and Processes of Biotechnology Short Questions and Answers

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(1) Restriction endonucleases are enzymes which
[A] Make cuts at specific positions within the DNA molecule
[B] Recognize a specific nucleotide sequence for binding of DNA ligase
[C] Restrict the actions of the enzyme DNA polymerase
[D] Remove nucleotides from the ends of the DNA molecules
Answer: Make cuts at specific positions within the DNA molecule
(2) Stirred-tank bioreactors have been designed for
[A] Addition of preservatives to the products
[B] Purification of the product
[C] Ensuring anaerobics conditions in the culture vessel
[D] Availability of oxygen throughout the process
Answer: Availability of oxygen throughout the process
(3) Which of the following are used in gene cloning ?
[A] Nucleoids
[B] Lomasomes
[C] Mesosomes
[D] Plasmids
Answer: Plasmids
(4) In genetic engineering, a DNA segment (Gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
i) Bacterium
ii) Plasmid
iii) Plasmodium
iv) Bacteriophage
[A] (i),(ii) & (iv)
[B] (i) only
[C] (i) & (iii)
[D] (ii) & (iv)
Answer: (ii) & (iv)
(5) Given below is a simple of a portion of DNA strand giving the base sequence on the opposite strands. What is so specialshown in it ?
5’ GAATTC 3’
3’ CTTAAG 5’
[A] Replication completed
[B] Deletion mutation
[C] Start condon at the 5’ end
[D] Plindromic sequence of base pairs
Answer: Plindromic sequence of base pairs
(6) There is a restriction endomolecules called Eco RI. What does “co” part in it stand for ?
[A] Colon
[B] Coelom
[C] Coenzyme
[D] Coli
Answer: Coli
(7) Agarose extracted from sea weeds is used in
[A] Spectrophotometry
[B] Tissue culture
[C] PCR
[D] Gel electrophoresis
Answer: Gel electrophoresis
(8) Which one of the following techniques made it possible to genetically engineer living organisms ?
[A] Recombinant DNA techniques
[B] X-ray diffraction
[C] Heavier isotope labeling
[D] Hybridizatiion
Answer: Recombinant DNA techniques
(9) The given figure is the diagrammatic representation of the E.Coli vector pBR322. Which one of the given options correctly identifies its certain components(s) ?
[A] Ori-original restriction enzyme
[B] Rop-reduced osmotic pressure
[C] Hin d III, Eco RI selectable markers
[D] AmpR , tetR – antibiotic resistance genes
Answer: AmpR , tetR – antibiotic resistance genes
(10) PCR and restriction fragment length polymorphism are the methods for
[A] Study of enzymes
[B] Genetic transformations
[C] DNA sequencing
[D] Genetic fingerprints
Answer: Genetic fingerprints

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