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NCERT - Biotechnology & its Applications Questions and Answers of Class 12 Biology | GkSeries

(1) Who is the father of tissue culture?
[1] Bonner
[2] Haberlandt
[3] Laibach
[4] Gautheret
Answer: Haberlandt
(2) Hormone pair required for a callus to differentiate are
[1] auxin and cytokinin
[2] auxin and ethylene
[3] auxin and absiccic acid
[4] cytokinins and gibberllin
Answer: auxin and cytokinin

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(3) Cybrids are produced by
[1] Fusion of two different nuclei from two different species
[2] Fusion of two same nuclei from same species
[3] Nucleus of one species but cytoplasm from both the parent species
[4] None of the above
Answer: Nucleus of one species but cytoplasm from both the parent species
(4) Part of plant used for culturing is called
[1] Scion
[2] Explant
[3] Stock
[4] Callus
Answer: Explant
(5) To obtain haploid plant, we culture
[1] Entire anther
[2] Nucleus
[3] Embryo
[4] Apical bud
Answer: Entire anther
(6) Which vector is mostly used in crop improvement?
[1] Plasmid
[2] Cosmid
[3] Phasmid
[4] Agrobacterium
Answer: Agrobacterium
(7) Golden rice is a transgenic crop of the future with the following improved trait
[1] Insect resistance
[2] High protein content
[3] High vitamin A content
[4] High lysine content
Answer: High vitamin A content
(8) Maximum application of animal cell culture technology today is in the production of
[1] Insulin
[2] Interferons
[3] Edible proteins
[4] Vaccines
Answer: Vaccines
(9) Two bacteria found to be very useful in genetic engineering experiments are
[1] Nitrobacter and Azotobacter
[2] Rhizobium and Diplococcus
[3] Nitrosomonas and Kliebsiella
[4] Escherichia and Agrobacterium
Answer: Escherichia and Agrobacterium
(10) In order to obtain virus free plants through tissue culture the best method is
[1] meristem culture
[2] protoplast culture
[3] anther culture
[4] embryo rescue
Answer: meristem culture
(11) Which of the following plant cell will show totipotency?
[1] Xylem vessels
[2] Sieve tube
[3] Meristem
[4] Cork cells
Answer: Meristem
(12) Growth hormone producing apical dominance is
[1] Auxin
[2] Gibberellin
[3] Ethylene
[4] Cytokinin
Answer: Auxin
(13) The most widely used chemical for protoplast fusion, as fusogens, is
[1] Manitol
[2] Sorbitol
[3] Mannol
[4] Poly ethylene glycol (PEG)
Answer: Poly ethylene glycol (PEG)
(14) Synthetic seed is produced by encapsulating somatic embryo with
[1] sodium chloride
[2] sodium alginate
[3] sodium acetate
[4] sodium nitrate
Answer: sodium alginate
(15) What is true for monoclonal antibodies?
[1] These antibodies obtained from one parent and for one antigen
[2] These obtained from many parents and for many antigens
[3] These obtained from different parents and for one antigen
[4] These obtained from one parent and for many antigens
Answer: These obtained from different parents and for one antigen

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