NCERT - Biotechnology Principles and Processes Questions and Answers of Class 12 Biology | GkSeries

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Questions
1 Rising of dough is due to:
1 Multiplication of yeast
2 Production of CO2
3 Emulsification
4 Hydrolysis of wheat flour starch into sugars.

Answer: Production of CO2
2 Restriction’ in Restriction enzyme refers to:
1 Cleaving of phosphodiester bond in DNA by the enzyme
2 Cutting of DNA at specific position only
3 Prevention of the multiplication of bacteriophage in bacteria
4 All of the above

Answer: Prevention of the multiplication of bacteriophage in bacteria
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3 While isolating DNA from bacteria, which of the following enzymes is not used?
1 Lysozyme
2 Ribonuclease
3 Deoxyribonuclease
4 Protease

Answer: Deoxyribonuclease
4 Significance of ‘heat shock’ method in bacterial transformation is to facilitate:
1 Binding of DNA to the cell wall
2 Uptake of DNA through membrane transport proteins
3 Uptake of DNA through transient pores in the bacterial cell wall
4 Expression of antibiotic resistance gene

Answer: Uptake of DNA through transient pores in the bacterial cell wall
5 Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
1 Laboratory flask of largest capacity
2 A stirred-tank bioreactor without in-lets and out-lets
3 A continuous culture system
4 Any of the above

Answer: A continuous culture system
6 Which of the following statements does not hold true for restriction enzyme?
1 It recognises a palindromic nucleotide sequence
2 It is an endonuclease
3 It is isolated from viruses
4 It produces the same kind of sticky ends in different DNA molecules

Answer: It is isolated from viruses
7 In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1 Size only
2 Charge only
3 Charge to size ratio
4 All of the above

Answer: Size only
8 The transfer of genetic material from one bacterium to another through the mediation of a vector like virus is termed as:
1 Transduction
2 Conjugation
3 Transformation
4 Translation

Answer: Transduction
9 Which of the following bacteria is not a source of restriction endonuclease?
1 Haemophilus influenzae
2 Escherichia coli
3 Agrobacterium tumefaciens
4 Bacillius amyloli

Answer: Agrobacterium tumefaciens
10 Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
1 Denaturation of template DNA
2 Annealing of primers to template DNA
3 Extension of primer end on the template DNA
4 All of the above

Answer: Extension of primer end on the template DNA
11 Who among the following was awarded the Nobel Prize for the development of PCR technique?
1 Herbert Boyer
2 Hargovind Khurana
3 Kary Mullis
4 Arthur Kornberg

Answer: Kary Mullis
12 A recombinant DNA molecule can be produced in the absence of the following:
1 Restriction endonuclease
2 DNA ligase
3 DNA fragments
4 E.coli

Answer: E.coli
13 The technique of DNA fingerprinting was perfected by
1 Stahl
2 Beadle and Tatum
3 Franklin
4 Alec Jeffreys

Answer: Alec Jeffreys
14 Restriction endonuclease (RE) was discovered by
1 Meselson
2 Cohen
3 Arber
4 Watson

Answer: Arber
15 Examples for clones
1 Monozygotic identical twins
2 Vegetative reproducing organism
3 Sexually reproducing organisms
4 All except C

Answer: All except C

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