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NCERT - Biotechnology Principles and Processes Questions and Answers of Class 12 Biology | GkSeries

31 Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein
[1] Binds with epithelial cells of midgut of the insect pest ultimately killing it
[2] Does not kill the carrier bacterium which is itself resistant to this toxin
[3] Is activated by acid pH of the foregut of the insect pest
[4] Is coded by several genes including the gene cry
Answer: Binds with epithelial cells of midgut of the insect pest ultimately killing it
32 A dividing and undifferentiated mass of cells is called
[1] Callus
[2] Embryo
[3] Explant
[4] Zygote
Answer: Callus

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33 What is true about Bt toxin?
[1] Bt protein exists as active toxin in the Bacillus
[2]
[3] The concerned Bacillus has antitoxins
[4] The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication
Answer: The concerned Bacillus has antitoxins
34 Which of the following enzyme joints the okazaki fragments?
[1] DNA polymerase
[2] DNA ligase
[3] Helicase
[4] Restriction endonuclease
Answer: DNA ligase
35 Which one of the following is a case of wrong matching?
[1] Micropropagation-In vitro production of plants in large numbers
[2] Callus - Unorganized mass of cells produced in tissue culture
[3] Somatic hybridization - Fusion of two diverse cells
[4] Vector DNA - Site for t-RNA synthesis
Answer: Vector DNA - Site for t-RNA synthesis
36 Which part would be most suitable for raising virus-free plants for micropropagation?
[1] Meristem
[2] Node
[3] Bark
[4] Vascular tissue
Answer: Meristem
37 Advancement in genetic engineering has been possible due to the discovery of
[1] Oncogenes
[2] Transposons
[3] Exonucleases
[4] Restriction endonuclease
Answer: Restriction endonuclease
38 Which of the following features should be present in vector?
[1] It should replicate autonomously
[2] It should have its own origin of replication
[3] It should possess restriction enzyme cleavage sites
[4] All of these
Answer: All of these
39 Agarose extracted from sea weeds finds use in
[1] Gel electrophoresis
[2] Spectrophotometry
[3] Tissue culture
[4] PCR
Answer: Gel electrophoresis
40 For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of
[1] Silicon or Platinum
[2] Gold or Tungsten
[3] Silver or Platinum
[4] Platinum or Zinc
Answer: Gold or Tungsten
41 Which one of the following is a correct statement?
[1] The anticoagulant hirudin is being produced from transgenic Brassica napus seeds
[2] Somatic hybridization involves fusion of two complete plant cells carrying desired genes
[3] "Bt" in "Bt-cotton" indicates that it is a genetically modified organism produced through biotechnology
[4] "Flavr Savr" variety of tomato has enhanced the production of ethylene which improves its taste
Answer: The anticoagulant hirudin is being produced from transgenic Brassica napus seeds
42 Which of the following is a genetic vector?
[1] Plasmid
[2] Phage
[3] Cosmid
[4] All of these
Answer: All of these
43 Continuous addition of sugars in 'fed batch' fermentation is done to
[1] Degrade sewage
[2] Produce methane
[3] Obtain antibiotics
[4] Purify enzymes
Answer: Purify enzymes
44 The linking of antibiotic resistance gene with the plasmid vector became possible with
[1] Endonucleases
[2] DNA ligase
[3] Exonucleases
[4] DNA polymerase
Answer: DNA ligase
45 Bacillus thuringiensis is used to control
[1] Nematodes
[2] Fungal pathogens
[3] Bacterial pathogens
[4] Insect pests
Answer: Insect pests

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