NCERT - MCQs on Biotechnology Principles and Processes of Class 12 Biology

61 Genetic engineering has been successfully used for producing

[1] Transgenic models for studying new treatments for certain cardiac diseases

[2] Transgenic mice for testing safety of polio vaccine before use in humans

[3] Animals like bulls for farm work as they have super power

[4] Transgenic Cow-Rosie which produces high fat milk for making ghee

Answer: Transgenic mice for testing safety of polio vaccine before use in humans

62 Which one contains four pyrimidine bases?

[1] GATCAATGC

[2] GCUAGACAA

[3] UAGCGGUAA

[4] TGCCTAACG

Answer: GATCAATGC

63 Which of the following is correctly matched?

[1] Ligase - Molecular scissors

[2] Thermus aquaticus - Bt-gene

[3] Agrobacterium tumefaciens - Tumour

[4] Hind II - Plasmid vector

Answer: Agrobacterium tumefaciens - Tumour

64 The specific triplet of nitrogen bases on the transfer RNA is called

[1] Codon

[2] Anticodon

[3] Genetic code

[4] none of the above

Answer: Anticodon

65 Restriction endonucleases:

[1] Are synthesized by bacteria as part of their defense mechanism

[2] Are used for in vitro DNA synthesis

[3] Are used in genetic engineering for ligation of two DNA molecules

[4] Are present in mammalian cells for degradation of DNA when the cell dies

Answer: Are synthesized by bacteria as part of their defense mechanism

66 Restriction endonucleases are used in genetic engineering and theyRestriction endonucleases are used in genetic engineering and they

[1] cut DNA at various sites

[2] join DNA segments

[3] cut RNA at specific sites

[4] cut DNA at specific sites

Answer: cut DNA at specific sites

67 DNA fingerprinting was discovered by

[1] James Watson

[2] Alec Jeffreys

[3] Frederick Fanger

[4] Hargobind Khorana

Answer: Alec Jeffreys

68 Which of the following enzymes are used to join bits of DNA?

[1] DNA polymerase

[2] Primase

[3] Ligase

[4] Endonuclease

Answer: Ligase

69 Sterilization of tissue culture medium is done by

[1] mixing the medium with antifungal agents

[2] filtering the medium through fine sieve

[3] autoclaving of medium at 120° for 15 min

[4] keeping the medium at –20°C

Answer: autoclaving of medium at 120° for 15 min

70 Which one is found only in RNA?

[1] Cytosine

[2] Adenine

[3] Uracil

[4] Guanine

Answer: Uracil

71 The term gene was coined by

[1] Mc Clintock

[2] Morgan

[3] Johannsen

[4] De Duve

Answer: Johannsen

72 Biolistics (gene-gun) is suitable for

[1] Constructing recombinant DNA by joining with vectors

[2] Transformation of plant cells

[3] Disarming pathogen vectors

[4] DNA finger printing

Answer: Transformation of plant cells

73 Restriction endonucleases are used in genetic engineering, because

[1] they can degrade harmful proteins

[2] they can join DNA fragments

[3] they can cut DNA at specific base sequences

[4] they can cut DNA at variable sites

Answer: they can cut DNA at specific base sequences

74 Which one of the following bacteria has found extensive use in genetic engineering work in plants?

[1] Bacillus coagulans

[2] Xanthomonas citri

[3] Clostridium septicum

[4] Agrobacterium tumefaciens

Answer: Agrobacterium tumefaciens

75 DNA is found in

[1] Chromosomes

[2] Plastids

[3] Mitochondria

[4] All of the above

Answer: All of the above

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