Ion Exchange Chromatography MCQs | Ion Exchange Chromatography Multiple Choice Questions and Answers

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Questions
1 Chromatography is generally used for the separation of mixtures based on the differences in their
A Shape
B Size
C Structure
D All Of Above

Answer: All Of Above
2 Ion-exchange chromatography is used for the separation of
A polar molecules
B non polar molecules
C both of above
D none of above

Answer: polar molecules
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3 Which polymer is often used for matrix materials in Affinity chromatography?
A Agarose
B Cellulose
C Dextrose
D All Of Above

Answer: All Of Above
4 Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme-substrate?
A Adsorption chromatography
B Ion –exchange chromatography
C Affinity chromatography
D Gel-permeation chromatography

Answer: Affinity chromatography
5 HPLC is an abbreviation for
A High Profit Liquid Chromatography
B High Pressure Liquid Chromatography
C Higher Performance Low Chromatography
D Higher Profit Low Chromatography

Answer: High Pressure Liquid Chromatography
6 Protein purification refers to the
A Purification of proteins
B Separation of proteins from other biomolecules
C Separation of a particular protein from other contaminating proteins
D all of these

Answer: Separation of a particular protein from other contaminating proteins
7 Primary steps in protein purification includes
A Homogenization
B Differential centrifugation
C Solubilisation
D All Of These

Answer: All Of These
8 The use of insulin hormone to purify its receptor is an example of
A Ion exchange chromatography
B Affinity chromatography
C Gel filtration chromatography
D Ligand mediated chromatography

Answer: Affinity chromatography
9 In anion exchange chromatography,
A the column contains negatively charged beads where positively charged proteins bind
B the column contains positively charged beads where negatively charged proteins bind
C the column contains both positive and negatively charged beads where proteins bind depending on their net charge
D all of these

Answer: the column contains positively charged beads where negatively charged proteins bind
10 A combination of paper chromatography and electrophoresis involves
A partition chromatography
B electrical mobility of the ionic species
C both (1) and (2)
D none of these

Answer: both (1) and (2)
11 In reverse phase chromatography, the stationary phase is made
A non-polar
B polar
C either non-polar or polar
D none of these

Answer: non-polar
12 Ion exchange chromatography is based on the
A electrostatic attraction
B electrical mobility of ionic species
C adsorption chromatography
D partition chromatography

Answer: electrostatic attraction
13 The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)
A V = V0 + kDVi
B V = V0/Vi
C V = V0 – kDVi
D None of the above

Answer: V = V0 + kDVi
14 In gas chromatography, the basis for separation of the components of the volatile material is the difference in
A partition coefficients
B conductivity
C molecular weight
D molarity

Answer: partition coefficients
15 In gel filtration chromatography, separation of proteins are based on their
A size and net charge
B size and shape
C size and specific affinity
D shape and net charge

Answer: size and shape
16 In which of the following separation method where proteins are separated on the basis of their net charge
A Affinity chromatography
B Ion exchange chromatography
C dialysis
D None of the above

Answer: Ion exchange chromatography
17 Which of the following separation method is suited for a protein sample with large differences in molecular mass
A dialysis
B salting out process
C density gradient centrifugation
D rate zonal centrifugation

Answer: rate zonal centrifugation
18 Salting out process involves
A precipitation of proteins using ammonium sulphate
B precipitation of proteins using copper sulphate
C precipitation of proteins using sodium chloride
D none of these

Answer: precipitation of proteins using ammonium sulphate
19 Which of the following detergent is commonly used to release integral proteins from its membranes?
A urea
B dimethyl sulphoxide
C triton X 100
D cyanogen bromide

Answer: triton X 100
20 Protein separation techniques are often based on the following properties except
A solubility of the protein
B viscosity of the protein
C charge of protein
D specific binding affinity of the protein

Answer: viscosity of the protein

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