Ion Exchange Chromatography Objective Type Questions and Answers

Questions

21 Which technique is much superior in terms of the speed, efficiency, sensitivity and ease of operation?

A Adsorption Chromatography

B Ion –exchange Chromatography

C High Performance Liquid Chromatography

D All Of Above

View Answer

Answer: High Performance Liquid Chromatography

22 Which is not generally used for separation of proteins, as proteins get denatured by it?

A Reverse Phase Chromatography

B Ion –exchange chromatography

C Affinity chromatography

D Gel-permeation chromatography

View Answer

Answer: Reverse Phase Chromatography

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23 Reversed-phase chromatography uses a stationary phase which is ………… in nature.

A Hydrophilic

B Hydrophobic

C Both of above

D All Of Above

View Answer

Answer: Hydrophobic

24 In Gel Permeation Chromatography which molecules will be eluted last?

A Small molecules

B Intermediate molecules

C Larger molecules

D All of these

View Answer

Answer: Small molecules

25 In Ion-exchange chromatography diffusion of the molecule through the matrix of the exchanger is dependent upon

A the degree of cross-linkages of the exchanger

B the ionic strength of the buffer

C both of above

D none of above

View Answer

Answer: both of above

26 Thin layer chromatography is

A partition chromatography

B electrical mobility of ionic species

C adsorption chromatography

D all of these

View Answer

Answer: adsorption chromatography

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