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Free download in PDF Ion Exchange Chromatography Multiple Choice Questions and Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams of B.Sc, M.Sc, Life-Science, Microbiloggy, Bio-Technology etc. These short solved questions or quizzes are provided by Gkseries.
(1)
Chromatography is generally used for the separation of mixtures based on the differences in their
[A]
Shape
[B]
Size
[C]
Structure
[D]
All Of Above
(2)
Ion-exchange chromatography is used for the separation of
[A]
polar molecules
[B]
non polar molecules
[C]
both of above
[D]
none of above
(3)
Which polymer is often used for matrix materials in Affinity chromatography?
[A]
Agarose
[B]
Cellulose
[C]
Dextrose
[D]
All Of Above
(4)
Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme-substrate?
[A]
Adsorption chromatography
[B]
Ion –exchange chromatography
[C]
Affinity chromatography
[D]
Gel-permeation chromatography
Answer: Affinity chromatography
(5)
HPLC is an abbreviation for
[A]
High Profit Liquid Chromatography
[B]
High Pressure Liquid Chromatography
[C]
Higher Performance Low Chromatography
[D]
Higher Profit Low Chromatography
Answer: High Pressure Liquid Chromatography
(6)
Protein purification refers to the
[A]
Purification of proteins
[B]
Separation of proteins from other biomolecules
[C]
Separation of a particular protein from other contaminating proteins
[D]
all of these
Answer: Separation of a particular protein from other contaminating proteins
(7)
Primary steps in protein purification includes
[A]
Homogenization
[B]
Differential centrifugation
[C]
Solubilisation
[D]
All Of These
(8)
The use of insulin hormone to purify its receptor is an example of
[A]
Ion exchange chromatography
[B]
Affinity chromatography
[C]
Gel filtration chromatography
[D]
Ligand mediated chromatography
Answer: Affinity chromatography
(9)
In anion exchange chromatography,
[A]
the column contains negatively charged beads where positively charged proteins bind
[B]
the column contains positively charged beads where negatively charged proteins bind
[C]
the column contains both positive and negatively charged beads where proteins bind depending on their net charge
[D]
all of these
Answer: the column contains positively charged beads where negatively charged proteins bind
(10)
A combination of paper chromatography and electrophoresis involves
[A]
partition chromatography
[B]
electrical mobility of the ionic species
[C]
both (1) and (2)
[D]
none of these
(11)
In reverse phase chromatography, the stationary phase is made
[A]
non-polar
[B]
polar
[C]
either non-polar or polar
[D]
none of these
(12)
Ion exchange chromatography is based on the
[A]
electrostatic attraction
[B]
electrical mobility of ionic species
[C]
adsorption chromatography
[D]
partition chromatography
Answer: electrostatic attraction
(13)
The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)
[A]
V = V0 + kDVi
[B]
V = V0/Vi
[C]
V = V0 – kDVi
[D]
None of the above
(14)
In gas chromatography, the basis for separation of the components of the volatile material is the difference in
[A]
partition coefficients
[B]
conductivity
[C]
molecular weight
[D]
molarity
Answer: partition coefficients
(15)
In gel filtration chromatography, separation of proteins are based on their
[A]
size and net charge
[B]
size and shape
[C]
size and specific affinity
[D]
shape and net charge
(16)
In which of the following separation method where proteins are separated on the basis of their net charge
[A]
Affinity chromatography
[B]
Ion exchange chromatography
[C]
dialysis
[D]
None of the above
Answer: Ion exchange chromatography
(17)
Which of the following separation method is suited for a protein sample with large differences in molecular mass
[A]
dialysis
[B]
salting out process
[C]
density gradient centrifugation
[D]
rate zonal centrifugation
Answer: rate zonal centrifugation
(18)
Salting out process involves
[A]
precipitation of proteins using ammonium sulphate
[B]
precipitation of proteins using copper sulphate
[C]
precipitation of proteins using sodium chloride
[D]
none of these
Answer: precipitation of proteins using ammonium sulphate
(19)
Which of the following detergent is commonly used to release integral proteins from its membranes?
[A]
urea
[B]
dimethyl sulphoxide
[C]
triton X 100
[D]
cyanogen bromide
(20)
Protein separation techniques are often based on the following properties except
[A]
solubility of the protein
[B]
viscosity of the protein
[C]
charge of protein
[D]
specific binding affinity of the protein
Answer: viscosity of the protein
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