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Ion Exchange Chromatography Quiz | Ion Exchange Chromatography Objective Type Questions and Answers

(21) Which technique is much superior in terms of the speed, efficiency, sensitivity and ease of operation?
[A] Adsorption Chromatography
[B] Ion –exchange Chromatography
[C] High Performance Liquid Chromatography
[D] All Of Above
Answer: High Performance Liquid Chromatography
(22) Which is not generally used for separation of proteins, as proteins get denatured by it?
[A] Reverse Phase Chromatography
[B] Ion –exchange chromatography
[C] Affinity chromatography
[D] Gel-permeation chromatography
Answer: Reverse Phase Chromatography

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(23) Reversed-phase chromatography uses a stationary phase which is ………… in nature.
[A] Hydrophilic
[B] Hydrophobic
[C] Both of above
[D] All Of Above
Answer: Hydrophobic
(24) In Gel Permeation Chromatography which molecules will be eluted last?
[A] Small molecules
[B] Intermediate molecules
[C] Larger molecules
[D] All of these
Answer: Small molecules
(25) In Ion-exchange chromatography diffusion of the molecule through the matrix of the exchanger is dependent upon
[A] the degree of cross-linkages of the exchanger
[B] the ionic strength of the buffer
[C] both of above
[D] none of above
Answer: both of above
(26) Thin layer chromatography is
[A] partition chromatography
[B] electrical mobility of ionic species
[C] adsorption chromatography
[D] all of these
Answer: adsorption chromatography

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