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Molecular Biology Techniques Quiz | Molecular Biology Techniques Objective Type Questions and Answers

(21) In E. Coli, Inactivation Of Cellular DNA Methylase Enzyme Causes Severe Mutation In The Genomic DNA. Which Of The Following DNA Repair Mechanism Would Be Most Probably Inhibited?
[A] Mismatch Repair
[B] Nucleotide Excision Repair
[C] Double Strand Break Repair
[D] Base Excision Repair
Answer: Mismatch Repair
(22) In Northern Hybridization Probe Hybridization Forms
[A] RNA:DNA Hybrid
[B] DNA:DNA Hybrid
[C] Both A And B
[D] None Of These
Answer: Both A And B

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(23) All Are Differences In Procedure Between Northern And Southern Hybridization Except
[A] Intially Fragments Are Separated By Electrophoresis In Northern Hybridization
[B] DNA Denaturation Is Required Before Blotting In Sothern Hybridization
[C] DBM Membrane Is Used In Northern Hybridization
[D] RNA:DNA Hybrids Are Formed In Northern Hybridization
Answer: Intially Fragments Are Separated By Electrophoresis In Northern Hybridization
(24) In Western Blotting
[A] Polyacrylamide Gel Is Commonly Used
[B] Agarose Gel Is Commonly Used
[C] Both A And B
[D] High Resolution Gels
Answer: Polyacrylamide Gel Is Commonly Used
(25) For Protein Detection, Most Commonly Used Probe Is
[A] Interferons
[B] Antigens
[C] Lectin
[D] Antibody
Answer: Antibody
(26) DNA Helicase Enzyme Involved In Base Excision Repair Mechanism Is______.
[A] DNA Helicase III
[B] DNA Helicase IV
[C] DNA Helicase I
[D] DNA Helicase II
Answer: DNA Helicase III
(27) Xeroderma Pigmentosum In Human Is Associated With A Mutation In
[A] Photoreactivation
[B] Mismatch Repair
[C] Nucleotide Excision Repair
[D] Base Excision Repair
Answer: Nucleotide Excision Repair
(28) Which Of The Following Statement Is Correct?
[A] Photoreactivation Is Present In All Organism
[B] Photoreactivation Is Present Only In Bacteria
[C] Photoreactivation Is Absent In Human
[D] None of these
Answer: Photoreactivation Is Absent In Human
(29) The Activity Of AP Endonuclease Is Involved In ________.
[A] Double Strand Break Repair
[B] Base Excision Repair
[C] Nucleotide Excision Repair
[D] Mismatch Repair
Answer: Base Excision Repair
(30) In Eukaryotes, The Mismatch Repair Mechanism Is Initiated And Directed By_____.
[A] Strand Specific Nicks
[B] Double Strand Breaks
[C] Methylated DNA Strand
[D] Acetylated DNA Strand
Answer: Strand Specific Nicks
31 The Two Chromophores Present In Photolyase Enzyme Are:
[A] FAD – And Folate
[B] FADH And Folate –
[C] NAD And FAD
[D] FAD And Folate
Answer: FAD – And Folate

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