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Free Download as PDF of the detailed solutions for class 12 Biology from NCERT or CBSE text book as per exam pattern, to help you in day to day learning. We provide all important questions and answers from chapter Biotechnology Principles and Processes of Biology.
(16)
The most important feature in a plasmid to be used as a vector is:
[1]
Origin of replication (ori)
[2]
Presence of a selectable marker
[3]
Presence of sites for restriction endonuclease
[4]
Its size
Answer: Origin of replication (ori)
(17)
An enzyme catalysing the removal of nucleotides from the ends of DNA is:
[1]
endonuclease
[2]
exonuclease
[3]
DNA ligase
[4]
Hind – II
(18)
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
[1]
Human gene may have intron which bacteria cannot process
[2]
Amino acid codons for humans and bacteria are different
[3]
Human protein is formed but degraded by bacteria
[4]
All of the above
Answer: Human gene may have intron which bacteria cannot process
(19)
Transgenic organisms means
[1]
An organism with all desirable characters
[2]
an organism with a particular mutated gene
[3]
an organism with foreign gene by genetic engineering
[4]
An organism with a number of mutated characters.
Answer: an organism with foreign gene by genetic engineering
(20)
Gene cloning means
[1]
Production of mutated genes
[2]
Production of wild genes
[3]
Production of dominant genes
[4]
production of large populations of desired DNA fragments
Answer: production of large populations of desired DNA fragments
21
Molecular scissors are
[1]
Ligase
[2]
Helicase
[3]
Restriction endonuclease
[4]
DNA polymerase
Answer: Restriction endonuclease
22
For DNA finger printing we need
[1]
cells from the tip of finger
[2]
[3]
cells from the reproductive organs
[4]
any cells from the fingers
Answer: cells from any part of the body
23
Which of the following has popularised the PCR (polymerase chain reactions)?
[1]
Easy availability of DNA template
[2]
Availability of synthetic primers
[3]
Availability of cheap deoxyribonucleotides
[4]
Availability of ‘Thermostable’ DNA polymerase
Answer: Availability of ‘Thermostable’ DNA polymerase
24
The role of DNA ligase in the construction of a recombinant DNA molecule is:
[1]
Formation of phosphodiester bond between two DNA fragments
[2]
Formation of hydrogen bonds between sticky ends of DNA fragments
[3]
Ligation of all purime and pyrimidine bases
[4]
None of the above
Answer: Formation of phosphodiester bond between two DNA fragments
25
Artificial synthesis of DNA without a template was first done by
[1]
Khorana
[2]
Geier
[3]
Meselson
[4]
Watson
26
Genetically modified DNA fragments are called
[1]
Recombinant DNA
[2]
S DNA
[3]
Mitochondrial DNA
[4]
None of these
27
Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
[1]
DNA can be seen in visible light
[2]
DNA can be seen without staining in visible light
[3]
Ethidium bromide stained DNA can be seen in visible light
[4]
Ethidium bromide stained DNA can be seen under exposure to UV light
Answer: Ethidium bromide stained DNA can be seen under exposure to UV light
28
An antibiotic resistance gene in a vector usually helps in the selection of:
[1]
Competent cells
[2]
Transformed cells
[3]
Recombinant cells
[4]
None of the above
Answer: Transformed cells
29
Totipotency means
[1]
Ability of a cell to grow into a complete plant
[2]
Ability of an animal cell to recombine with plant cell
[3]
Ability of a cell to grow into a complete individual
[4]
Ability of an animal cell to fully developed animal cell
Answer: Ability of a cell to grow into a complete individual
30
Which one is a true statement regarding DNA polymerase used in PCR?
[1]
It is isolated from a virus
[2]
It remains active at high temperature
[3]
It is used to ligate introduced DNA in recipient cells
[4]
It serves as a selectable marker
Answer: It remains active at high temperature
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