Molecular Biology Techniques MCQs | Molecular Biology Techniques Multiple Choice Questions and Answers

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Questions
1 Southern Hybridization is
A Used To Identify A Specific DNA
B Used To Identify Both DNA And RNA
C Used To Identify A Specific Protein
D Used To Identify A Specific RNA

Answer: Used To Identify A Specific DNA
2 Applications Of Southern Blotting Includes
A Identification Of Transferred Genes
B DNA Fingerprinting
C Preparation Of RFLP Maps
D All Of These

Answer: All Of These
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3 First DNA Glycosylase Enzyme Discovered Is ___________.
A Thymine DNA Glycosylase
B Adenine DNA Glycosylase
C Methyl Adenine Glycosylase
D Uracil DNA Glycosylase

Answer: Uracil DNA Glycosylase
4 The Enzyme Photolyase Repair The Thymine Dimer In The DNA By __________ Mechanism.
A Free Radical Mechanism
B Adduct Formation
C Oxidation Mechanism
D Direct Bond Breaking

Answer: Free Radical Mechanism
5 The Function Of Uvrc In NER Mechanism Is_________.
A Catalyze The Incision At The 3’ End Of The Lesion
B Catalyze The Incision At The 5’ Side Of The Lesion
C To Identify The Lesion In The DNA
D Helicase Activity To Unwind The DNA

Answer: Catalyze The Incision At The 5’ Side Of The Lesion
6 The Catalytic Activity Of Photolyase Enzyme In The Bacterial Cells Is Activated By _____.
A Visible Light Near Blue Region
B Visible Light Near Red Region
C IR Light
D UV Light

Answer: Visible Light Near Blue Region
7 Photolyase Enzyme Involved In The Photoreactivation Can Remove ______.
A UV Induced Thymine Dimers
B Single Strand Breaks In DNA
C UV Induced Double Strand Breaks
D Mismatches In DNA

Answer: UV Induced Thymine Dimers
8 Which Of The Following Gene Is NOT Involved In Mismatch Repair System In Bacteria?
A Mutl
B Muts
C Muta
D None of these

Answer: Muta
9 Which Enzyme Is Responsible For Photoreactivation Of DNA:
A Photolyase
B Photo-Oxidase
C Photoligase
D Photoreductase

Answer: Photolyase
10 Which Of The Following DNA Repair Mechanism Is Known As The ‘Cut And Patch Mechanism’?
A Nucleotide Excision Repair
B Mismatch Repair
C Photoreactivation
D None of these

Answer: Nucleotide Excision Repair
11 Which Of The Following Enzyme(S) Is Involved Base Excision Repair?
A DNA Glycosylase
B AP Endonuclease
C AP Exonuclease
D Both (A) And (B)

Answer: Both (A) And (B)
12 The Technique Used To Identify Specific DNA Sequence In Bacterial Colonies Is
A Western Blotting
B Dot Blot Technique
C In Situ Hybridization
D Colony Hybridization

Answer: Colony Hybridization
13 Which Of The Following Statements Are True Regarding Southern Blotting
A DNA-DNA Hybridization Is The Basis
B The Transfer Of DNA Fragments From Gel To Nitrocellulose Membrane Is Called Blotting
C Developed By E.M.Southern
D All Of These

Answer: All Of These
14 The Technique Used To Detect The Presence Of DNA Or RNA In A Non-Fractionated DNA Sample Is
A Dot Blot Technique
B Western Blotting
C Colony Hybridization
D None of these

Answer: Dot Blot Technique
15 Northern Hybridization Is
A Used To Identify Both DNA And RNA
B Used To Identify A Specific RNA
C Used To Identify A Specific Protein
D Used To Identify A Specific DNA

Answer: Used To Identify A Specific RNA
16 Southern Hybridization is
A Used To Identify Both DNA And RNA
B Used To Identify A Specific DNA
C Used To Identify A Specific RNA
D Used To Identify A Specific Protein

Answer: Used To Identify A Specific DNA
17 For Glycoproteins, Most Commonly Used Probe Is
A Interferons
B Antigens
C Lectin
D Antibody

Answer: Lectin
18 ELISA Is Based On
A Lectin- Antibody Interaction
B Antigen-Protein Intercation
C Antigen-Antibody Interaction
D All Of These

Answer: Antigen-Antibody Interaction
19 The Technique Used To Locate Specific Genes In Chromosomes Is
A Colony Hybridization
B Dot Blot Technique
C Western Blotting
D In Situ Hybridization

Answer: In Situ Hybridization
20 The Separation Technique Of Charged Molecules Under The Influence Of Electric Current Is Called
A Western Blotting
B Colony Hybridization
C Dot Blot Technique
D Electrophoresis

Answer: Electrophoresis

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