Molecular Biology Techniques Objective Type Questions and Answers

Questions

21 In E. Coli, Inactivation Of Cellular DNA Methylase Enzyme Causes Severe Mutation In The Genomic DNA. Which Of The Following DNA Repair Mechanism Would Be Most Probably Inhibited?

A Mismatch Repair

B Nucleotide Excision Repair

C Double Strand Break Repair

D Base Excision Repair

View Answer

Answer: Mismatch Repair

22 In Northern Hybridization Probe Hybridization Forms

A RNA:DNA Hybrid

B DNA:DNA Hybrid

C Both A And B

D None Of These

View Answer

Answer: Both A And B

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23 All Are Differences In Procedure Between Northern And Southern Hybridization Except

A Intially Fragments Are Separated By Electrophoresis In Northern Hybridization

B DNA Denaturation Is Required Before Blotting In Sothern Hybridization

C DBM Membrane Is Used In Northern Hybridization

D RNA:DNA Hybrids Are Formed In Northern Hybridization

View Answer

Answer: Intially Fragments Are Separated By Electrophoresis In Northern Hybridization

24 In Western Blotting

A Polyacrylamide Gel Is Commonly Used

B Agarose Gel Is Commonly Used

C Both A And B

D High Resolution Gels

View Answer

Answer: Polyacrylamide Gel Is Commonly Used

25 For Protein Detection, Most Commonly Used Probe Is

A Interferons

B Antigens

C Lectin

D Antibody

View Answer

Answer: Antibody

26 DNA Helicase Enzyme Involved In Base Excision Repair Mechanism Is______.

A DNA Helicase III

B DNA Helicase IV

C DNA Helicase I

D DNA Helicase II

View Answer

Answer: DNA Helicase III

27 Xeroderma Pigmentosum In Human Is Associated With A Mutation In

A Photoreactivation

B Mismatch Repair

C Nucleotide Excision Repair

D Base Excision Repair

View Answer

Answer: Nucleotide Excision Repair

28 Which Of The Following Statement Is Correct?

A Photoreactivation Is Present In All Organism

B Photoreactivation Is Present Only In Bacteria

C Photoreactivation Is Absent In Human

D None of these

View Answer

Answer: Photoreactivation Is Absent In Human

29 The Activity Of AP Endonuclease Is Involved In ________.

A Double Strand Break Repair

B Base Excision Repair

C Nucleotide Excision Repair

D Mismatch Repair

View Answer

Answer: Base Excision Repair

30 In Eukaryotes, The Mismatch Repair Mechanism Is Initiated And Directed By_____.

A Strand Specific Nicks

B Double Strand Breaks

C Methylated DNA Strand

D Acetylated DNA Strand

View Answer

Answer: Strand Specific Nicks

31 The Two Chromophores Present In Photolyase Enzyme Are:

A FAD – And Folate

B FADH And Folate –

C NAD And FAD

D FAD And Folate

View Answer

Answer: FAD – And Folate

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