Molecular Biology Techniques Quiz | Molecular Biology Techniques Objective Type Questions and Answers

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Questions
21 In E. Coli, Inactivation Of Cellular DNA Methylase Enzyme Causes Severe Mutation In The Genomic DNA. Which Of The Following DNA Repair Mechanism Would Be Most Probably Inhibited?
A Mismatch Repair
B Nucleotide Excision Repair
C Double Strand Break Repair
D Base Excision Repair

Answer: Mismatch Repair
22 In Northern Hybridization Probe Hybridization Forms
A RNA:DNA Hybrid
B DNA:DNA Hybrid
C Both A And B
D None Of These

Answer: Both A And B
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23 All Are Differences In Procedure Between Northern And Southern Hybridization Except
A Intially Fragments Are Separated By Electrophoresis In Northern Hybridization
B DNA Denaturation Is Required Before Blotting In Sothern Hybridization
C DBM Membrane Is Used In Northern Hybridization
D RNA:DNA Hybrids Are Formed In Northern Hybridization

Answer: Intially Fragments Are Separated By Electrophoresis In Northern Hybridization
24 In Western Blotting
A Polyacrylamide Gel Is Commonly Used
B Agarose Gel Is Commonly Used
C Both A And B
D High Resolution Gels

Answer: Polyacrylamide Gel Is Commonly Used
25 For Protein Detection, Most Commonly Used Probe Is
A Interferons
B Antigens
C Lectin
D Antibody

Answer: Antibody
26 DNA Helicase Enzyme Involved In Base Excision Repair Mechanism Is______.
A DNA Helicase III
B DNA Helicase IV
C DNA Helicase I
D DNA Helicase II

Answer: DNA Helicase III
27 Xeroderma Pigmentosum In Human Is Associated With A Mutation In
A Photoreactivation
B Mismatch Repair
C Nucleotide Excision Repair
D Base Excision Repair

Answer: Nucleotide Excision Repair
28 Which Of The Following Statement Is Correct?
A Photoreactivation Is Present In All Organism
B Photoreactivation Is Present Only In Bacteria
C Photoreactivation Is Absent In Human
D None of these

Answer: Photoreactivation Is Absent In Human
29 The Activity Of AP Endonuclease Is Involved In ________.
A Double Strand Break Repair
B Base Excision Repair
C Nucleotide Excision Repair
D Mismatch Repair

Answer: Base Excision Repair
30 In Eukaryotes, The Mismatch Repair Mechanism Is Initiated And Directed By_____.
A Strand Specific Nicks
B Double Strand Breaks
C Methylated DNA Strand
D Acetylated DNA Strand

Answer: Strand Specific Nicks
31 The Two Chromophores Present In Photolyase Enzyme Are:
A FAD – And Folate
B FADH And Folate –
C NAD And FAD
D FAD And Folate

Answer: FAD – And Folate

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