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21
In eukaryotes, the consensus promoter sequences (TATA box) that are required for initiation of transcription is generally present
A
10 nucleotide upstream of transcription start site (TSS)
22
In eukaryotes, the RNA synthesis process is more complex than prokaryotes. The RNA synthesis process is regulated by chromatin structure, upstream and downstream sequences, binding partners, etc. Which of the following is TRUE regarding the transcription process in eukaryotes:
A
Most actively transcribed genes are found in a loosely relaxed form of chromatin called euchromatin
B
The most inactive segment of DNA is found in compact chromatin structure called heterochromatin
C
Histone modification such as methylation, acetylation regulate the RNA transcription by modulating chromatin structure
23
Rifamycin is an antibiotic used for the treatment of tuberculosis. It binds to .... subunit of RNA polymerase and inhibits the initiation of transcription.
24
The termination of transcription occurs in both rho-dependent and rho-independent manner. Which of the following is NOT true regarding the termination of transcription
A
rho proteins recognize C-rich region near 3'end of the newly synthesized RNA
B
rho-independent termination occurs when the transcription reaches the palindromic structure leading to the formation of hairpins
C
rho protein competes with RNA polymerase for binding to nucleotides
26
RNA polymerase catalyzes the synthesis of RNA by addition nucleotide monophosphate and release of pyrophosphate for nucleotide triphosphate. RNA polymerase
28
The RNA polymerase is a multi-subunit enzyme that recognizes a consensus nucleotide sequence (promoter region) upstream of the transcription start site. In prokaryotes, the consensus promoter sequence consists of 5-TATAAT-3' also known as
