Principles and Processes of Biotechnology MCQs | Principles and Processes of Biotechnology Multiple Choice Questions and Answers

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Questions
1 A singe strand of nucleic acid tagged with a radioactive molecules is called
A Vector
B Selectable marker
C Plasmid
D Probe

Answer:Probe
2 Which one of the following is a case of wrong matching ?
A Somatic Hybridization- Fusion of two diverse cells
B Vector DNA- Site for tRNA synthesis
C Micropropagation- in vitro production of plants in large numbers
D Callus- Unorganised mass of cells produced in tissue culture

Answer:Vector DNA- Site for tRNA synthesis
3 Which one is a true statement regarding DNA polymerase used in PCR ?
A It is used to ligate introduced DNA in recipients cells
B It serves as a selectable marker
C It is isolated from a virus
D It remains active at high temperature

Answer: It remains active at high temperature
4 For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of
A Silver or platinum
B Platinum or zinc
C Silicon or platinum
D Gold or trungsten

Answer: Gold or trungsten
5 Biolistics (gene-gun) is suitable
A Disarming pathogen vector
B Transformation of plant cells
C Constructing recombinant DNA by joining with vectors
D DNA fingerprinting

Answer: Transformation of plant cells
6 In genetic engineering, the antibiotics are used
A As selectable markers
B To select healthy vectors
C As sequence from where replication starts
D To keep the culture free of infection

Answer:As selectable markers
7 Which one of the following represents a palindromic sequence in DNA ?
A 5’-GAATTC-3 3’-CTTAAG-5’
B 5’-CCAATG-3’
C 3’-GAATCC-5’
D 5’-CATTAG-3’ 3’-GATAAC-5’

Answer: 5’-GAATTC-3 3’-CTTAAG-5’
8 The colonies of recombinant bacteria appear white in contrast to blue colonies of non- recombinant bacteria because of
A Insertional inactivation of alpha galactosidese in recombinant bacteria
B Inactivation of glycosides enzyme in recombinant bacteria
C Non-recombinant bacteria containing beta galactosidase
D Insertional inactivation of alpha galactosidase

Answer:Non-recombinant bacteria containing beta galactosidase
9 Which of the following is not correctly matched for the organism and its cell wall degrading enzyme ?
A Algae - Methylase
B Fungi - Chitinase
C Bacteria - Lysozyme
D Plant cells – Cellulase

Answer: Algae - Methylase
10 DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
A Electrophoresis
B Restriction mapping
C Centrifugation
D Polymerase chain reaction

Answer: Electrophoresis
11 An analysis of chromosomal DNA using the southern hybridization technique does not use
A Electrophoresis
B Blotting
C Autoradiography
D PCR

Answer:PCR
12 In vitro clonal propagation in plants is characterized by
A PCR and RAPD
B Northern blotting
C Electrophoresis and HPLC
D Microscopy

Answer: PCR and RAPD
13 Which vector can be clone only a small fragment of DNA ?
A Bacterial artificial chromosome
B Yeast artificial chromosome
C Plasmid
D Cosmid

Answer: Plasmid
14 Commonly used vectors for human genome sequencing are
A T- DNA
B BAC and YAC
C Expression vectors
D T/A cloning vectors

Answer: BAC and YAC
15 Which of the following is a plasmid ?
A pBR322
B BamH-I
C Hind-III
D EcoRI

Answer:pBR322
16 Restriction endonucleases are must widely used in recombinant DNA technology. They are obtained from
A Bacteriophages
B Bacterial cells
C Plasmids
D All Prokaryotie Cells

Answer: Bacterial cells
17 Viral genome incorporated into host DNA is called
A Prophase
B Prophage
C Bacteriophage
D None of these

Answer: Prophage
18 Two microbes found to be very useful in genetic engineering are
A Crown gall bacterium and Conorhabditis elegens
B Escherichia coli to Agrobacterium tumifaciens
C Vibria choleraeand a tailed bacteriphage
D Dipococens species and psendomonasap

Answer:Escherichia coli to Agrobacterium tumifaciens
19 Who disconnected recombinant DNA technoledge ?
A Har Gobind Khorana
B James Watson & Francis Crick
C Stanly Cohen & Herbert Boyer
D Watter Sutton

Answer: Stanly Cohen & Herbert Boyer
20 Find out the wrong statement ?
A Mobile genetic element, Transposons were visualized by Barbara Mc Clintock
B Udder cell a somatic cell is used to produce the cloned sheep by nuclear transplantation method
C Dr. Ian Wilmut produced a cloned sheep called Dolley
D DNA ligases are used to cleave a DNA molecule

Answer: DNA ligases are used to cleave a DNA molecule

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