9 Principles and Processes of Biotechnology Objective Type Questions and Answers | Principles and Processes of Biotechnology Quiz

Principles and Processes of Biotechnology MCQs | Principles and Processes of Biotechnology Objective Type Questions and Answers

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Questions
1 The DNA fragments have sticky ends due to
A Endonuclease
B Unpaired bases
C Calcium ions
D Free methylation

Answer:Unpaired bases
2 Plasmids are used as cloning vectors for which of the following reasons?
A Can be multiplied in culture
B Self-replication in bacterial cells
C Can be multiplied in laboratories with the help of enzymes
D Replicate freely outside bacterial cells

Answer: Can be multiplied in laboratories with the help of enzymes
3 The human genome project was launched in the year
A 1980
B 1973
C 1990
D 1989

Answer: 1990
4 The vaccines prepared through recombinant DNA technology are
A Third generation vaccines
B First-generation vaccines
C Second-generation vaccines
D None

Answer:Third generation vaccines
5 Which is a genetically modified crop?
A Bt-cotton
B Bt-brinjal
C Golden rice
D All

Answer: All
6 PCR technique was invented by
A Karry Mullis
B Boyer
C Sanger
D Cohn

Answer:Karry Mullis
7 The first transgenic plant to be produced is
A Brinjal
B Tobacco
C Rice
D Cotton

Answer:Tobacco
8 RNA interference helps in
A Cell proliferation
B Micropropagation
C Cell defence
D Cell differentiation

Answer:Cell defence
9 Which of the following is the quality of improved transgenic basmati rice?
A Gives high yield but no characteristic aroma
B Gives high yield and is rich in vitamin A
C Does not require chemical fertilizers and growth hormones
D Resistant to insects and diseases

Answer:Gives high yield and is rich in vitamin A
10 The first clinical application of gene therapy over a 4 year old girl was for
A Adenosine deaminase deficiency
B Adenosine deficiency
C Growth deficiency
D Adenine deficiency

Answer: Adenosine deaminase deficiency
11 Excision and insertion of a gene is called
A Biotechnology
B Genetic engineering
C Cytogenetics
D Gene therapy

Answer: Genetic engineering
12 The expression of a transgene in the target tissue is identified by a
A Transgene
B Promoter
C Enhancer
D Reporter

Answer: Reporter
13 ———– is used as a vector for cloning into higher organisms
A Retrovirus
B Baculovirus
C Salmonella typhimurium
D Rhizopus nigricans

Answer: Retrovirus
14 Which bacterium is used in the production of insulin by genetic engineering?
A Saccharomyces
B Rhizobium
C Escherichia
D Mycobacterium

Answer: Escherichia
15 Restriction endonuclease
A Synthesizes DNA
B Cuts the DNA molecule randomly
C Cuts the DNA molecule at specific sites
D Restricts the synthesis of DNA inside the molecules

Answer: Cuts the DNA molecule at specific sites
16 Gel electrophoresis is used for
A Construction of recombinant DNA by joining with cloning vectors
B Isolation of DNA molecules
C Cutting of DNA into fragments
D Separation of DNA fragments according to their size

Answer:Separation of DNA fragments according to their size
17 The linking of antibiotic resistance gene with the plasmid vector become possible with
A DNA polymerase
B Exonucleases
C DNA ligase
D Endonucleases

Answer:DNA ligase
18 Polyethylene glycol method is used for
A Biodiesel production
B Seedless fruit production
C Energy production from swage
D Gene transfer without a vector

Answer:Gene transfer without a vector
19 Which one of the following is used as vector for cloning genes into higher organisms ?
A Baculovirus
B Salmonella typhimurium
C Rhizopus nigricans
D Retrovirus

Answer:Retrovirus
20 DNA or RNA segment tagged with a radioactive molecules is called
A Vector
B Probe
C Clone
D Plasmid

Answer:Probe

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