Principles and Processes of Biotechnology Short Questions and Answers | Principles and Processes of Biotechnology Quiz

1 Restriction endonucleases are enzymes which

A Make cuts at specific positions within the DNA molecule

B Recognize a specific nucleotide sequence for binding of DNA ligase

C Restrict the actions of the enzyme DNA polymerase

D Remove nucleotides from the ends of the DNA molecules

View Answer

Answer:Make cuts at specific positions within the DNA molecule

2 Stirred-tank bioreactors have been designed for

A Addition of preservatives to the products

B Purification of the product

C Ensuring anaerobics conditions in the culture vessel

D Availability of oxygen throughout the process

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Answer:Availability of oxygen throughout the process

3 Which of the following are used in gene cloning ?

A Nucleoids

B Lomasomes

C Mesosomes

D Plasmids

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Answer:Plasmids

4 In genetic engineering, a DNA segment (Gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
i) Bacterium
ii) Plasmid
iii) Plasmodium
iv) Bacteriophage

A (i),(ii) & (iv)

B (i) only

C (i) & (iii)

D (ii) & (iv)

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Answer:(ii) & (iv)

5 Given below is a simple of a portion of DNA strand giving the base sequence on the opposite strands. What is so specialshown in it ?
5’ GAATTC 3’
3’ CTTAAG 5’

A Replication completed

B Deletion mutation

C Start condon at the 5’ end

D Plindromic sequence of base pairs

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Answer: Plindromic sequence of base pairs

6 There is a restriction endomolecules called Eco RI. What does “co” part in it stand for ?

A Colon

B Coelom

C Coenzyme

D Coli

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Answer:Coli

7 Agarose extracted from sea weeds is used in

A Spectrophotometry

B Tissue culture

C PCR

D Gel electrophoresis

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Answer:Gel electrophoresis

8 Which one of the following techniques made it possible to genetically engineer living organisms ?

A Recombinant DNA techniques

B X-ray diffraction

C Heavier isotope labeling

D Hybridizatiion

View Answer

Answer:Recombinant DNA techniques

9 The given figure is the diagrammatic representation of the E.Coli vector pBR322. Which one of the given options correctly identifies its certain components(s) ?