Principles and Processes of Biotechnology MCQs | Principles and Processes of Biotechnology Multiple Choice Questions with Answers

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Questions
1 One of the key factors which makes the plasmid the vector in genetic engineering is that
A It is resistant to antibiotics
B It is resistant to restriction enzymes
C Its ability to carry a foreign gene
D Its ability to cause infection in the host

Answer:Its ability to carry a foreign gene
2 Which of the following is used as a best genetic vector in plants
A Bacillus thurienglnesis
B Agrobacterium thumifaciens
C Psendomonas putida
D All of the above

Answer: Agrobacterium thumifaciens
3 The polymerase chain reaction is a technique that
A It is used for in vivo replication of DNA
B It is used for in vivo synthesis of mRNA
C It is used for in vitro synthesis of mRNA
D It is used for in vitro replication of specific DNA sequence using thermostable DNA polymerase.

Answer:It is used for in vitro replication of specific DNA sequence using thermostable DNA polymerase.
4 The construction of the first recombinant DNA was done by using the native plasmid of
A E coli
B Salmonella typhimurium
C Bacillus thuringiensis
D Agrobacterium

Answer: Salmonella typhimurium
5 Gel electrophoresis is used for
A Construction of recombinant DNA by joining with cloning vectors
B Isolation of DNA molecules
C Cutting of DNA is to fragments
D Separation of DNA fragments according to their size

Answer: Separation of DNA fragments according to their size
6 Significant of ‘heat shoch’ method in bacterial transformation is to facilitate ?
A Binding of DNA to the cell wall
B Update of DNA through membrane transport proteins
C Update of DNA through transient pores in the bacterial cell wall
D Expression of antibiotic resistant gene

Answer: Update of DNA through transient pores in the bacterial cell wall
7 Which of the following palindromic bare sequences in DNA can be easily cut at about the middle by some particular restriction enzyme.
A 5’CACGTA 3’ : 3’CTCAGT 5’
B 5’CGTTCG 3’ : 3’ATGGTA 5’
C 5’GATATC 3’ : 3’CTACTA 5’
D 5’GAATTC 3’ : CTTAAG 5’

Answer: 5’GAATTC 3’ : CTTAAG 5’
8 Agarose extracted from sea weeds in used in
A Spectrophotometry
B Tissue culture
C PCR
D Gel electrophoresis

Answer:Gel electrophoresis
9 Which one of the following technique made it possible to genetically engineered living organisms ?
A Recombinant DNA technique
B X-ray diffraction
C Heavier isotope labelling
D Hybridization

Answer: Recombinant DNA technique
10 There is a restriction endonucleare called EcoRI. What does ‘co’ part in it stand for ?
A Colon
B Coelom
C Coenzyme
D Coli

Answer: Coli
11 A single stand of nucleic acid tagged tagged with a radioactive molecule is called
A Vector
B Selectable marker
C Plasmid
D Probe

Answer: Probe
12 For transformation, micro particles coated with DNA to be bombared with gene gun are made up of
A Silver or Platinum
B Platinum or Zine
C Silicon or Platinum
D Gold or Tungsten

Answer: Gold or Tungsten
13 Biolistics (Gene-gun) is suitable for
A Disarming pathogen vectors
B Transformation of plant cells
C Constructing recombinant DNA by joining with vectors
D DNA fingerprinting

Answer: Transformation of plant cells
14 In genetic engineering, the antibiotics are used
A As selectable markers
B To select healthy vectors
C As sequence from where replication starts
D To keep the culture free from infection

Answer: As selectable markers
15 Restriction enzymes were discovered by
A Smith and Nathans
B Alexander Fleming
C Berg
D None

Answer: Smith and Nathans
16 Bacteria protect themselves from viruses by fragmenting viral DNA with
A Ligase
B Endonuclease
C Exonuclease
D Gyrase

Answer:Endonuclease
17 Klenow fragment is derived from
A DNA Ligase
B DNA Pol-I
C DNA Pol-II
D Reverse Transcriptase

Answer: DNA Pol-I
18 Southern blotting is
A Attachment of probes to DNA fragments
B Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
C Comparison of DNA fragments to two sources
D Transfer of DNA fragments to electrophoretic gel from cellulose membrane

Answer: Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
19 ELISA is
A Using radiolabelled second antibody
B Usage of RBCs
C Using complement-mediated cell lysis
D Addition of substrate that is converted into a coloured end product

Answer: Addition of substrate that is converted into a coloured end product
20 The Golden Rice variety is rich in
A Vitamin C
B Β-carotene and ferritin
C Biotin
D Lysine

Answer:Β-carotene and ferritin

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