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Molecular Basis of Inheritance MCQs | Molecular Basis of Inheritance Multiple Choice Questions with Answers

(1) The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA?
[A] Stacked between histone molecules
[B] Binds to the nucleotide base
[C] Intercalated between the stacked bases
[D] Binds to the phosphodiester backbone
Answer: Intercalated between the stacked bases
(2) Pulse field gel electrophoresis separates DNA molecules of size ___________
[A] 10 – 20 bp
[B] 20 – 30 Kb
[C] 30 – 50 Kb
[D] 40 – 50 bp
Answer: 30 – 50 Kb
(3) Which of the following will migrate faster? The condition is the molecular weight of the following is equal.
[A] Supercoiled circular DNA
[B] Nicked circular DNA
[C] Single stranded DNA
[D] Double stranded DNA
Answer: Supercoiled circular DNA
(4) Agarose can be extracted from which of the following?
[A] Gracilaria esculentus
[B] Lycazusican esculentum
[C] Ficum benghalensis
[D] Agrostis stolonifera
Answer: Gracilaria esculentus
(5) Electrophoresis cannot be used to separate _______________
[A] DNA
[B] RNA
[C] Amino acid
[D] Protein
Answer: Amino acid
(6) Which of the following is not a character of polyacrylamide gel?
[A] Inert
[B] Ionic strength
[C] Stable over a wide range of pH
[D] Separate upto a few 100 bp of DNA
Answer: Separate upto a few 100 bp of DNA
(7) Pulse field gel electrophoresis was developed by ____________
[A] Collins and John
[B] Kary Mullis
[C] Patrick O’ Farrell
[D] Schwartz and Cantor
Answer: Schwartz and Cantor
(8) What is the condition required to observe B form of DNA?
[A] Low humidity
[B] High humidity
[C] Low temperature
[D] High temperature
Answer: High humidity
(9) What is the condition required to observe A form of DNA?
[A] Low humidity
[B] High humidity
[C] Low temperature
[D] High temperature
Answer: Low humidity
(10) How many bases are there in per turn of the B form of DNA?
[A] 8 bp
[B] 9 bp
[C] 10 bp
[D] 11 bp
Answer: 10 bp
(11) Which of the following is not a character of A DNA?
[A] Short and broad
[B] Left handed helix
[C] Narrow minor groove
[D] Broad and shallow major groove
Answer: Left handed helix
(12) How many bases are there in per turn of the A form of DNA?
[A] 11 bp
[B] 10 bp
[C] 12 bp
[D] 8 bp
Answer: 11 bp
(13) Which of the following is not a character of B form of DNA?
[A] Longer and thinner
[B] Right-handed helix
[C] Narrow minor groove
[D] Flat major groove
Answer: Flat major groove
(14) In a right handed DNA the glycosidic bond is in syn configuration.
[A] True
[B] False
Answer: False
(15) In the Z form of DNA the fundamental repeating unit is __________
[A] Purine – pyrimidine nucleotide
[B] Purine – pyrimidine dinucleotide
[C] Purine – pyrimidine tri-nucleotide
[D] Purine – pyrimidine tetra-nucleotide
Answer: Purine – pyrimidine tetra-nucleotide
(16) What is the factor responsible for the left-handed helical conformation in Z form of DNA?
[A] Syn conformation at pyrimidine
[B] Anti conformation at pyrimidine
[C] Syn conformation at purine
[D] Anti conformation at purine
Answer: Anti conformation at purine
(17) In higher salt concentration the DNA helix assumes the right-handed conformation.
[A] True
[B] False
Answer: False
(18) The two strands of cccDNA can be easily separated without any breakage of bonds.
[A] True
[B] False
Answer: False
(19) Passing of one strand through the other in order to get separated is known as _______
[A] Twist
[B] Linking number
[C] Writhing number
[D] Toroid
Answer: Linking number
(20) Linking number is always a ___________
[A] Whole number
[B] Prime number
[C] Integer
[D] Even number
Answer: Integer

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