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Free download in PDF Molecular Basis of Inheritance Short Questions with Answers for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams like NEET, AIIMS, JIPMER etc. These short solved questions or quizzes are provided by Gkseries./p>
(1)
During electrophoresis denaturation of the double stranded DNA is brought about by __________
[A]
Treatment with alkali
[B]
Application of current
[C]
Treatment with EtBr
[D]
Application of heat
Answer: Treatment with alkali
(2)
In case of northern blotting the hybridization occurs between two RNA molecules.
(3)
Northern blotting is performed for ____________
[A]
Determining the size of DNA
[B]
Determining the size of RNA
[C]
Quantification of RNA
[D]
Sequencing of RNA
Answer: Quantification of RNA
(4)
The process by which a probe is used to screen a library is known as ___________
[A]
Hybridization
[B]
Southern blotting
[C]
Colony hybridization
[D]
Western blotting
Answer: Colony hybridization
(5)
PCR is a DNA amplifying in vivo method.
(6)
Which of the following is a mismatch?
[A]
Polymerase – Taq polymerase
[B]
Template – double stranded DNA
[C]
Primer – oligonucleotide
[D]
Synthesis – 5’ to 3’ direction
Answer: Template – double stranded DNA
(7)
Annealing of primer is facilitated by complementary region.
(8)
Primer used for the process of polymerase chain reaction are ______________
[A]
Single stranded DNA oligonucleotide
[B]
Double stranded DNA oligonucleotide
[C]
Single stranded RNA oligonucleotide
[D]
Double stranded RNA oligonucleotide
Answer: Single stranded DNA oligonucleotide
(9)
Polymerase used for PCR is extracted from _____________
[A]
Escherichia coli
[B]
Homo sapiens
[C]
Thermus aquaticus
[D]
Saccharomyces cerevisiae
Answer: Thermus aquaticus
(10)
How many DNA duplex is obtained from one DNA duplex after 4 cycles of PCR?
[A]
4
[B]
8
[C]
16
[D]
32
(11)
At what temperature do denaturation of DNA double helix takes place?
[A]
60˚
[B]
54˚
[C]
74˚
[D]
94˚
(12)
At what temperature do annealing of DNA and primer takes place?
[A]
42˚
[B]
54˚
[C]
74˚
[D]
96˚
(13)
The temperature at which DNA synthesis takes place is 74˚C.
(14)
The type of DNA amplification where the region of DNA amplified lies on either side of a known segment __________
[A]
RT – PCR
[B]
Anchored – PCR
[C]
Inverse – PCR
[D]
Nested – PCR
(15)
Primer complementary to regions used in inverse PCR is _____________
[A]
3’ end of unknown region
[B]
5’ end of unknown region
[C]
3’ end of known region
[D]
5’ end of known region
Answer: 3’ end of known region
(16)
With respect to target DNA used in inverted PCR which of the following is not true?
[A]
Restricted segment
[B]
Blunt ended segment
[C]
Intact known segment
[D]
Flanked unknown segment flanked on either side
Answer: Blunt ended segment
(17)
The DNA concentration in inverse PCR is kept low.
(18)
The linear fragment used for inverted PCR has known ends _____________
(19)
With respect to RAPD which of the following is false?
[A]
10 bases long
[B]
G/C rich
[C]
Has inverted repeats
[D]
Radom sequences are used
Answer: Has inverted repeats
(20)
Polymorphism in RAPD is observed because ______________
[A]
DNA used is from different chromosomes of same species
[B]
DNA used is from same chromosomes of same species
[C]
DNA used is from different chromosomes of different species
[D]
DNA used is from complementary chromosomes of same species
Answer: DNA used is from different chromosomes of different species
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