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Free download in PDF Very Short Questions and Answers on Molecular Basis of Inheritance for competitive exams. These short objective type questions with answers are very important for Board exams as well as competitive exams like NEET, AIIMS, JIPMER etc. These short solved questions or quizzes are provided by Gkseries./p>
(1)
RAPDs cannot be used for PCR amplification.
(2)
RAPDs are much more convenient markers than RFLPs.
(3)
The inheritance pattern of RAPD is _______________
[A]
Dominant
[B]
Recessive
[C]
Codominant
[D]
Random
(4)
RAPDs can be used to detect multiple alleles of a marker.
(5)
Reverse transcriptase produces DNA from RNA.
(6)
Which of the following serves as the first primer in RT – PCR for eukaryotic RNA?
[A]
Oligo A
[B]
Oligo T
[C]
Oligo G
[D]
Oligo C
(7)
In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________
[A]
5’ end of RNA
[B]
3’ end of RNA
[C]
3’ end of cDNA
[D]
5’ end of cDNA
(8)
The digestion of mRNA during RT – PCR is carried out by the enzyme ____________
[A]
Exonuclease
[B]
RNase H
[C]
Bal 31
[D]
Endonuclease
(9)
How many primers are used in the process of reverse transcriptase amplification?
(10)
From which organism is the enzyme reverse transcriptase isolated?
[A]
Bacteria
[B]
Fungi
[C]
Virus
[D]
Prions
(11)
The cDNA library provides several advantages over RT – PCR.
(12)
________________ is a modification of RT – PCR.
[A]
Overlap extension PCR
[B]
Inverse PCR
[C]
Thermal cycle sequencing PCR
[D]
RACE
(13)
Which of the following is not required for DNA sequencing?
[A]
Restriction digestion
[B]
Electrophoresis
[C]
Cloning
[D]
Polymerase chain reaction
(14)
End labeled DNA sequencing is known as dideoxy method of sequencing.
(15)
The 32P is added at the 3’ end by polynucleotidyl kinase.
(16)
The end labeled fragment is cleaved in how many pieces?
(17)
Restriction digestion is the only process to achieve sequencing by the Maxem and Gilbert method.
(18)
The denatured strands cannot be separated.
(19)
What are the basic base-specific cleavage sites used in Maxem and Gilbert method?
[A]
A, T, G, C
[B]
C, T, A+G, T+C
[C]
A, G, A+T, G+C
[D]
G, C, A+G, C+T
(20)
Which step is not involved in base-specific cleavage of DNA fragment?
[A]
Modification of concerned base
[B]
Removal of modified base from DNA strand
[C]
Induction of random strand break
[D]
End labeled DNA fragments of variable lengths produced
Answer: Induction of random strand break
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