Molecular Basis of Inheritance MCQs | Molecular Basis of Inheritance Multiple Choice Questions with Answers

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Questions
1 The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA?
A Stacked between histone molecules
B Binds to the nucleotide base
C Intercalated between the stacked bases
D Binds to the phosphodiester backbone

Answer:Intercalated between the stacked bases
2 Pulse field gel electrophoresis separates DNA molecules of size ___________
A 10 – 20 bp
B 20 – 30 Kb
C 30 – 50 Kb
D 40 – 50 bp

Answer: 30 – 50 Kb
3 Which of the following will migrate faster? The condition is the molecular weight of the following is equal.
A Supercoiled circular DNA
B Nicked circular DNA
C Single stranded DNA
D Double stranded DNA

Answer: Supercoiled circular DNA
4 Agarose can be extracted from which of the following?
A Gracilaria esculentus
B Lycazusican esculentum
C Ficum benghalensis
D Agrostis stolonifera

Answer:Gracilaria esculentus
5 Electrophoresis cannot be used to separate _______________
A DNA
B RNA
C Amino acid
D Protein

Answer: Amino acid
6 Which of the following is not a character of polyacrylamide gel?
A Inert
B Ionic strength
C Stable over a wide range of pH
D Separate upto a few 100 bp of DNA

Answer:Separate upto a few 100 bp of DNA
7 Pulse field gel electrophoresis was developed by ____________
A Collins and John
B Kary Mullis
C Patrick O’ Farrell
D Schwartz and Cantor

Answer: Schwartz and Cantor
8 What is the condition required to observe B form of DNA?
A Low humidity
B High humidity
C Low temperature
D High temperature

Answer:High humidity
9 What is the condition required to observe A form of DNA?
A Low humidity
B High humidity
C Low temperature
D High temperature

Answer:Low humidity
10 How many bases are there in per turn of the B form of DNA?
A 8 bp
B 9 bp
C 10 bp
D 11 bp

Answer:10 bp
11 Which of the following is not a character of A DNA?
A Short and broad
B Left handed helix
C Narrow minor groove
D Broad and shallow major groove

Answer:Left handed helix
12 How many bases are there in per turn of the A form of DNA?
A 11 bp
B 10 bp
C 12 bp
D 8 bp

Answer: 11 bp
13 Which of the following is not a character of B form of DNA?
A Longer and thinner
B Right-handed helix
C Narrow minor groove
D Flat major groove

Answer: Flat major groove
14 In a right handed DNA the glycosidic bond is in syn configuration.
A True
B False

Answer: False
15 In the Z form of DNA the fundamental repeating unit is __________
A Purine – pyrimidine nucleotide
B Purine – pyrimidine dinucleotide
C Purine – pyrimidine tri-nucleotide
D Purine – pyrimidine tetra-nucleotide

Answer:Purine – pyrimidine tetra-nucleotide
16 What is the factor responsible for the left-handed helical conformation in Z form of DNA?
A Syn conformation at pyrimidine
B Anti conformation at pyrimidine
C Syn conformation at purine
D Anti conformation at purine

Answer:Anti conformation at purine
17 In higher salt concentration the DNA helix assumes the right-handed conformation.
A True
B False

Answer:False
18 The two strands of cccDNA can be easily separated without any breakage of bonds.
A True
B False

Answer:False
19 Passing of one strand through the other in order to get separated is known as _______
A Twist
B Linking number
C Writhing number
D Toroid

Answer: Linking number
20 Linking number is always a ___________
A Whole number
B Prime number
C Integer
D Even number

Answer: Integer

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