Molecular Basis of Inheritance MCQs | Molecular Basis of Inheritance Short Questions and Answers

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Questions
1 How does telomerase activity depend on age?
A Directly
B Inversely
C Remains the same
D Does not occur

Answer:Does not occur
2 In cancer telomerase activity __________
A Increases
B Decreases
C Remains constant
D Plays no role

Answer: Increases
3 During DNA cloning which of the following is not a crucial requirement?
A DNA inserts
B Vector
C Protein expression
D Molecular cutter

Answer: Protein expression
4 Transformation does not involve ____________
A Cutting
B Recombination
C Propagation
D Expression

Answer:Expression
5 Which is the most common organism considered for genetic manipulations?
A E. coli
B Saccharomyces cerevisiae
C Cyanobacteria
D Bacillus Subtilis

Answer: E. coli
6 Which of the following is an essential feature for being a perfect vector?
A Origin of replication
B Selectable marker
C Restriction site
D Virulent gene

Answer:Virulent gene
7 DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme.
A True
B False

Answer: True
8 The vector and the DNA insert are cut by different enzymes for convenience.
A True
B False

Answer:False
9 What is the major difference between cloning vectors and primary vectors?
A Selectable marker
B DNA inserts
C Presence of promoter
D Presence of two Ori

Answer:Presence of promoter
10 Which of the following is the primary use of an expression vector?
A DNA library
B DNA purification
C Protein production
D DNA cloning

Answer:Protein production
11 Under the influence of which ion does E. coli takes up plasmid from the environment?
A Nickel
B Copper
C Lead
D None of these

Answer:Copper
12 The process by which every type of transformant can be identified is __________
A Replica plating
B Hybridization
C Blotting
D Insertional inactivation

Answer: Insertional inactivation
13 Which of the following does not affect the formation of hybrid DNA?
A Ionic strength
B Pressure
C Temperature
D Homologous DNA

Answer: Pressure
14 Which of the following is not termed as hybridization?
A DNA and cDNA
B DNA and mRNA
C DNA from different species
D DNA from male and female of same species

Answer: DNA from male and female of same species
15 What is a probe?
A Chemically synthesized DNA
B Purified DNA
C Fragmented DNA duplex
D Either purified or synthesized single stranded DNA

Answer:Either purified or synthesized single stranded DNA
16 Why is a probe labeled?
A Improve visibility
B Improve stability
C Improve location identification
D Improve binding capability

Answer:Improve location identification
17 In how many ways a DNA can be labeled?
A 2
B 3
C 4
D 5

Answer:2
18 What is the function of polynucleotide kinase?
A Addition of γ – phosphate at 3’ – OH
B Addition of γ – phosphate at 5’ – OH
C Removal of γ – phosphate at 3’ – OH
D Removal of γ – phosphate at 5’ – OH

Answer:Addition of γ – phosphate at 5’ – OH
19 Fluorescent labeling is an important phenomenon in case of hybridization and manipulations in molecular biology.
A True
B False

Answer: False
20 Radiolabelling is generally brought about by addition of radioactive phosphorus.
A True
B False

Answer: True

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