Molecular Basis of Inheritance MCQs | Molecular Basis of Inheritance Short Questions with Answers

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Questions
1 During electrophoresis denaturation of the double stranded DNA is brought about by __________
A Treatment with alkali
B Application of current
C Treatment with EtBr
D Application of heat

Answer:Treatment with alkali
2 In case of northern blotting the hybridization occurs between two RNA molecules.
A True
B False

Answer:False
3 Northern blotting is performed for ____________
A Determining the size of DNA
B Determining the size of RNA
C Quantification of RNA
D Sequencing of RNA

Answer: Quantification of RNA
4 The process by which a probe is used to screen a library is known as ___________
A Hybridization
B Southern blotting
C Colony hybridization
D Western blotting

Answer:Colony hybridization
5 PCR is a DNA amplifying in vivo method.
A True
B False

Answer: False
6 Which of the following is a mismatch?
A Polymerase – Taq polymerase
B Template – double stranded DNA
C Primer – oligonucleotide
D Synthesis – 5’ to 3’ direction

Answer:Template – double stranded DNA
7 Annealing of primer is facilitated by complementary region.
A True
B False

Answer: True
8 Primer used for the process of polymerase chain reaction are ______________
A Single stranded DNA oligonucleotide
B Double stranded DNA oligonucleotide
C Single stranded RNA oligonucleotide
D Double stranded RNA oligonucleotide

Answer:Single stranded DNA oligonucleotide
9 Polymerase used for PCR is extracted from _____________
A Escherichia coli
B Homo sapiens
C Thermus aquaticus
D Saccharomyces cerevisiae

Answer:Thermus aquaticus
10 How many DNA duplex is obtained from one DNA duplex after 4 cycles of PCR?
A 4
B 8
C 16
D 32

Answer:16
11 At what temperature do denaturation of DNA double helix takes place?
A 60˚
B 54˚
C 74˚
D 94˚

Answer:94˚
12 At what temperature do annealing of DNA and primer takes place?
A 42˚
B 54˚
C 74˚
D 96˚

Answer:96˚
13 The temperature at which DNA synthesis takes place is 74˚C.
A True
B False

Answer: True
14 The type of DNA amplification where the region of DNA amplified lies on either side of a known segment __________
A RT – PCR
B Anchored – PCR
C Inverse – PCR
D Nested – PCR

Answer: Inverse – PCR
15 Primer complementary to regions used in inverse PCR is _____________
A 3’ end of unknown region
B 5’ end of unknown region
C 3’ end of known region
D 5’ end of known region

Answer:3’ end of known region
16 With respect to target DNA used in inverted PCR which of the following is not true?
A Restricted segment
B Blunt ended segment
C Intact known segment
D Flanked unknown segment flanked on either side

Answer:Blunt ended segment
17 The DNA concentration in inverse PCR is kept low.
A True
B False

Answer:True
18 The linear fragment used for inverted PCR has known ends _____________
A True
B False

Answer:True
19 With respect to RAPD which of the following is false?
A 10 bases long
B G/C rich
C Has inverted repeats
D Radom sequences are used

Answer: Has inverted repeats
20 Polymorphism in RAPD is observed because ______________
A DNA used is from different chromosomes of same species
B DNA used is from same chromosomes of same species
C DNA used is from different chromosomes of different species
D DNA used is from complementary chromosomes of same species

Answer: DNA used is from different chromosomes of different species

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