Molecular Basis of Inheritance MCQs | Molecular Basis of Inheritance Very Short Questions and Answers

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Questions
1 Which of the following does not affect DNA replication?
A Antiparallel nature of DNA
B End specificity of polymerase
C SSB protein
D Helicase

Answer:SSB protein
2 Who was the first person to analyse the process of replication and on which organism?
A Arthur Kornberg: E. coli
B John Cairns: E. coli
C Arthur Kornberg: Bacillus subtilis
D John Cairns: Bacillus subtilis

Answer: John Cairns: E. coli
3 In the case of a circular DNA synthesis how many replication forks are observed?
A 1
B 2
C 3
D 4

Answer: 2
4 Primer synthesis short stretches of DNA for replication.
A True
B False

Answer:False
5 Which of the following is not used for degrading RNA from RNA:DNA hybrid in replication?
A RNase A
B RNase H
C Polymerase I
D Exonuclease

Answer: RNase A
6 The gaps formed by the hydrolyzing the RNA from RNA:DNA hybrid is filled by DNA polymerase I and the nicks are ligated by T4 ligase
A True
B False

Answer:False
7 Which of the following is correctly matched with its subsequent role?
A Topoisomerase II- can remove both positive and negative supercoil in the DNA duplex
B Polymerase I- larger fragment responsible for exonuclease activity
C DnaA protein- responsible for “melting” of the DNA double helix during replication
D DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand

Answer: DnaA protein- responsible for “melting” of the DNA double helix during replication
8 Which of the following types of DNA polymerase has 3’→5’ exonuclease activity?
A DNA polymerase I
B DNA polymerase II
C DNA polymerase III
D DNA polymerase IV

Answer:DNA polymerase I
9 Choose the odd one out with respect to DNA polymerase III.
A dna E
B dna N
C dna Q
D dna B

Answer:dna B
10 DNA polymerase II can polymerase upto ______________ nucleotides per minute at 37˚C.
A 50
B 500
C 5000
D 50000

Answer:50
11 All mutation in polymerases is lethal except the mutation of polymerase III.
A True
B False

Answer:False
12 Polymerase I has the highest affinity for dNTPs.
A True
B False

Answer: False
13 Polymerase I has two fragments. The residue of larger fragment consists from ____________ residues.
A 1 – 234
B 234 – 658
C 324 – 928
D 234 – 928

Answer: 324 – 928
14 Which of the following is false about klenow fragment?
A Polymerization activity
B 3’→5’ exonuclease activity
C 5’→3’ exonuclease activity
D 324 – 928 residue of polymerase I

Answer: 3’→5’ exonuclease activity
15 DNA polymerase III has ___________ subunits. a) 4 b) c) d) View Answer Answer: d
A 4
B 6
C 8
D 10

Answer:10
16 Which of the following types of DNA polymerase does not take part in DNA repair?
A DNA polymerase I
B DNA polymerase II
C DNA polymerase III
D DNA polymerase IV

Answer:DNA polymerase III
17 How many active sites are present in the DNA polymerase to catalyze the addition of the four dNTPs?
A 1
B 2
C 3
D 4

Answer:1
18 How does the polymerase recognize the correct dNTP for an addition?
A Structure of dNTP
B Molecular weight of dNTP
C Purine and pyrimidine orientation
D Ability of hydrogen bond formation

Answer:Ability of hydrogen bond formation
19 DNA polymerase can distinguish between dNTPs and rNTPS due to ___________
A Structural differentiation
B Steric exclusion
C Steric hindrance
D Enzyme substrate mismatch

Answer: Steric exclusion
20 When we compare the structure of DNA polymerase to the structure of a body part, it resembles to _________________
A Right hand
B Left hand
C Right foot
D Left foot

Answer: Right hand

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