Molecular Basis of Inheritance MCQs | Very Short Questions with Answers on Molecular Basis of Inheritance

Sports GK Questions and Answers 2024 (Latest Updated)

Awards & Honours GK Questions 2024 (Latest Updated)

Questions
1 The process of electrophoresis is the key to sequencing.
A True
B False

Answer:True
2 The dideoxy method is also known as _____________
A Maxem and Gilbert method
B Autosequencing
C Sanger’s enzymatic sequencing
D Pyrosequencing

Answer: Sanger’s enzymatic sequencing
3 Which enzyme is used for the replication in case of Sanger’s method of sequencing?
A Polymerase I
B Smaller subunit polymerase I
C Polymerase III
D Larger subunit polymerase I

Answer: Larger subunit polymerase I
4 With respect to Sanger’s enzymatic method of sequencing pick the odd one out.
A Radioactive dideoxyribonucleotides
B Primers
C Klenow fragment
D Restriction digestion

Answer:Restriction digestion
5 The four reaction mixtures that were prepared, contained ____________
A dCTP, dTTP, dGTP, dATP
B dCTP, ddTTP, dGTP, ddATP
C ddCTP, dTTP, ddGTP, dATP
D dCTP, ddTTP, dGTP, dATP

Answer: dCTP, ddTTP, dGTP, dATP
6 ddNTP acts as the chain radioactive markers.
A True
B False

Answer:False
7 Strand separation for DNA sequencing is done to ____________
A sort the fragments according to their size
B sort the fragments according to their molecular weight
C obtain consecutive sequence as per termination
D obtain specific lanes of sequencing

Answer: obtain consecutive sequence as per termination
8 The position of band during electrophoresis indicates the dNTP used as a chain terminator in that mixture.
A True
B False

Answer:False
9 Automated sequencing is defined as _________
A Chain termination sequencing
B Radio labeled sequencing
C Real time fluorescence sequencing
D Pyrosequencing

Answer:Real time fluorescence sequencing
10 Automated sequencing uses _____________
A Enzymatic process
B Radiolabelling
C Chain termination
D Fluorescent tagging

Answer:Fluorescent tagging
11 No chain termination takes place in automated sequencing.
A True
B False

Answer:False
12 As different fluorescent tags affect the mobility of fragments thus only one type of tag is used.
A True
B False

Answer: False
13 With respect to automated sequencing which of the following is true?
A Detected by radioactive tags
B Multiple plane sequencing
C Horizontal scanning one sequence per lane
D Vertical reading software incorporation

Answer:Horizontal scanning one sequence per lane
14 Which of the following feature is required for the high sensitivity of the tags used for autosequencing?
A Strong absorption at UV spectrum
B Maximum emittion at different wavelengths
C Different mobility shifts
D Induce circularization of DNA fragments

Answer: Maximum emittion at different wavelengths
15 Automated sequencing is a real time one trial method of sequencing and is highly handy.
A True
B False

Answer:False
16 A third generation sequencing can sequence upto ____________nucleotides per day.
A 750000
B 75000
C 570000
D 57000

Answer:750000
17 Semiconservative nature of replication of eukaryotic chromosome was first demonstrated by _______________
A Walter Flemming on root tip cells of Vicia faba
B J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba
C Walter Flemming on root tip cells of Phaseolus vulgaris
D J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Phaseolus vulgaris

Answer:J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba
18 Pick the correct pair with respect to primers used in DNA replication.
A RNA primer- for prokaryotes only
B DNA primer-for eukaryotes only
C DNA primer- for both prokaryotes and eukaryotes
D RNA primer- for both prokaryotes and eukaryotes

Answer:RNA primer- for both prokaryotes and eukaryotes
19 Which of the following is correctly matched with its subsequent role?
A Topoisomerase II- can remove both positive and negative supercoil in the DNA duplex
B Polymerase I- larger fragment responsible for exonuclease activity
C DnaA protein- responsible for “melting” of the DNA double helix during replication
D DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand

Answer: DnaA protein- responsible for “melting” of the DNA double helix during replication
20 Replication fork is the junction between the two ___________
A Unreplicated DNA
B Newly synthesized DNA
C Newly separated DNA strands and newly synthesized DNA strands
D Newly separated DNA strands and the unreplicated DNA

Answer: Newly separated DNA strands and the unreplicated DNA

Random GK Questions

Q. The writ of Habeas Corpus is issued in the event of
Q. In a filament type light bulb most of electric power consumed appears as :
Q. An ECG shows the functioning of the
Q. In which one of the following states is Nagarjunasagar sanctuary located ?
Q. Antyodaya Programme is associated with
Q. Which one of the following is taiga biome ?
Assam Direct Recruitment Test Series

Teacher Eligibility Test

Assam Direct Recruitment Test Series