Molecular Basis of Inheritance MCQs | Very Short Questions and Answers on Molecular Basis of Inheritance

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Questions
1 RAPDs cannot be used for PCR amplification.
A True
B False

Answer:False
2 RAPDs are much more convenient markers than RFLPs.
A True
B False

Answer: True
3 The inheritance pattern of RAPD is _______________
A Dominant
B Recessive
C Codominant
D Random

Answer: Dominant
4 RAPDs can be used to detect multiple alleles of a marker.
A True
B False

Answer:False
5 Reverse transcriptase produces DNA from RNA.
A True
B False

Answer: False
6 Which of the following serves as the first primer in RT – PCR for eukaryotic RNA?
A Oligo A
B Oligo T
C Oligo G
D Oligo C

Answer:Oligo T
7 In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________
A 5’ end of RNA
B 3’ end of RNA
C 3’ end of cDNA
D 5’ end of cDNA

Answer:3’ end of cDNA
8 The digestion of mRNA during RT – PCR is carried out by the enzyme ____________
A Exonuclease
B RNase H
C Bal 31
D Endonuclease

Answer:RNase H
9 How many primers are used in the process of reverse transcriptase amplification?
A 1
B 2
C 3
D 4

Answer:2
10 From which organism is the enzyme reverse transcriptase isolated?
A Bacteria
B Fungi
C Virus
D Prions

Answer:Virus
11 The cDNA library provides several advantages over RT – PCR.
A True
B False

Answer:False
12 ________________ is a modification of RT – PCR.
A Overlap extension PCR
B Inverse PCR
C Thermal cycle sequencing PCR
D RACE

Answer: All of these
13 Which of the following is not required for DNA sequencing?
A Restriction digestion
B Electrophoresis
C Cloning
D Polymerase chain reaction

Answer: Cloning
14 End labeled DNA sequencing is known as dideoxy method of sequencing.
A True
B False

Answer: False
15 The 32P is added at the 3’ end by polynucleotidyl kinase.
A True
B False

Answer:False
16 The end labeled fragment is cleaved in how many pieces?
A 2
B 3
C 4
D 5

Answer:2
17 Restriction digestion is the only process to achieve sequencing by the Maxem and Gilbert method.
A True
B False

Answer:False
18 The denatured strands cannot be separated.
A True
B False

Answer:False
19 What are the basic base-specific cleavage sites used in Maxem and Gilbert method?
A A, T, G, C
B C, T, A+G, T+C
C A, G, A+T, G+C
D G, C, A+G, C+T

Answer: G, C, A+G, C+T
20 Which step is not involved in base-specific cleavage of DNA fragment?
A Modification of concerned base
B Removal of modified base from DNA strand
C Induction of random strand break
D End labeled DNA fragments of variable lengths produced

Answer: Induction of random strand break

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